結合光子晶體與微流道之檢測平台

Abstract

本計畫將嘗試整合微流道晶片及光子晶體感測器來開發一個可以快速處理檢測樣本之生物分子檢測平台。微流道晶片用來進行樣本的前置處理，包含微流道和過濾區，過濾區是做為生物實驗上過濾細胞及其他物質的用途，這些元件將被整合在一矽基板上，以期達到快速且自動化的樣本處理。在檢測部分，本團隊將開發高靈敏度光子晶體(Photonic crystal)，來達到免標定(label-free)感測。為了驗證檢測平台，本計畫將對細胞內的Beta actin蛋白質進行檢測，將細胞樣本注入所設計的檢測晶片中，經由過濾區過濾細胞碎片及雜質，待待測蛋白質流經檢測區並與抗體鍵結後，藉由觀察光子晶體共振波長的變化來量測細胞內的蛋白質。結果顯示，過濾區的微柱可以有效過濾細胞碎片與雜質，光子晶體針對Beta actin偵測極限為160 ng/ml。

The goal of this project is to develop a novel Lab-on-a-chip (LOC) detection system. The proposed system consists of a microfluidic subsystem and a label-free (LF) detection subsystem. The system can facilitate sample preparation and provide immediate detections. The microfluidic subsystem consists of several components including two liquid inlet which can inject the samples and reagents, and filters for cell debris purification after lysis. All these components will be fabricated on a silicon chip simultaneously. Regarding the detection subsystem, we developed a sensitive photonic crystal (PC) biosensor for LF detection of proteins. The PC functions as an optical filter where only a particular combination of wavelength/illumination angle can excite the structure resonance, resulting in a strong reflection, while other combinations of wavelengths and illumination angles are transmitted through. Through monitoring the shift of the reflected wavelength, the concentration of the analyte can be determined. The designed LOC system was demonstrated to detect the Beta-actin proteins inside the cell and simultaneously to filter the cell debris. The result indicates the detection limit of 160 ng/ml can be achieved without any prefiltering procedure.