標題: Highly sensitive color-indicating and quantitative biosensor based on cholesteric liquid crystal
作者: Hsiao, Yu-Cheng
Sung, Yu-Chien
Lee, Mon-Juan
Lee, Wei
影像與生醫光電研究所
Institute of Imaging and Biomedical Photonics
公開日期: 1-Dec-2015
摘要: Liquid crystal (LC)-based biosensors employ highly sensitive interfaces between the alignment layers and LCs to detect biomolecules and their interactions. Present techniques based on optical texture observation of the homeotropic-to-planar response of nematic LCs are limited by their quantitative reproducibility of results, indicating that both the accuracy and reliability of LC-based detection require further improvements. Here we show that cholesteric LC (CLC) can be used as a novel sensing element in the design of an alternative LC-based biosensing device. The chirality of the vertically anchored (VA) CLC was exploited in the detection of bovine serum albumin (BSA), a protein standard commonly used in protein quantitation. The color appearance and the corresponding transmission spectrum of the cholesteric phase changed with the concentration of BSA, by which a detection limit of 1 fg/ml was observed. The optical response of the VA CLC interface offers a simple and inexpensive platform for highly sensitive and naked-eye color-indicating detection of biomolecules, and, thus, may facilitate the development of point-of-care devices for the detection of disease-related biomarkers. (C) 2015 Optical Society of America
URI: http://dx.doi.org/10.1364/BOE.6.005033
http://hdl.handle.net/11536/129341
ISSN: 2156-7085
DOI: 10.1364/BOE.6.005033
期刊: BIOMEDICAL OPTICS EXPRESS
Volume: 6
Issue: 12
起始頁: 5033
結束頁: 5038
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