Detection of Multiple Embryo Growth Factors by Bead -Based Digital Microfluidic Chip in Embryo Culture Medium

Abstract

-In IVF treatment, the pregnancy rate of selective single embryo implantation can be improved by detection the concentration of embryo growth factors such as IL -to and TNF-a in embryo culture medium. In this study, we manufacture the high dielectric constant digital microfluidic chip and develop a Bead -based digital microfluidic immunoassay with low sample requirement, low limit of detection and rapid immunoassay. We establish the mouse TNF-a calibration curve and the requirement of sample is 520 nL, limit of detection is 1.55 pg/mL and total immunoassay time is 27 min. We also establish the Human IL -1(3 calibration curve and the requirement of sample is 520 nL, limit of detection is 0.0579 pg/mL and total immunoassay time is 21 min.