A Phototransistor-Based High-Sensitivity Biosensing System Using 650-nm Light

Abstract

A miniature optical biosensing system based on a PMOS phototransistor and absorption photometry is proposed. The phototransistor was manufactured in a standard 0.35-mu m CMOS process, and it exhibited a responsivity higher than 1000 A/W for 650-nm light. For biochemical applications, the TMB/H(2)O(2)/HRP method was adopted as a useful basis in our system. A sample volume of only 10 mu l was required to be dropped on the slide above the phototransistor. Experimental results demonstrated that a high sensitivity of 2.5 mu A/pM was achieved, and the minimum HRP concentration successfully detected was 2.7 pM. This detection limit is three orders of magnitude better than that of a lately reported silicon biosensor, and is even comparable to that of a commercial spectrophotometer.