Kinetic Studies and Developments of Quick Assay for Glucose Isomerase

Abstract

Due to significant commercial importance, the production of invert sugar and fructose, using glucose isomerase from a suitable microorganism is currently studied. A relative simple extension of Michaelis-Menten kinetics with incorporation of enzyme inactivation is formulated. Determinations of raction rate and its dependence on the stable variables, such as pH of solution, salt concentration, glucose quantity and isomerase quantity, are performed in this research.