LABEL-FREE SEQUENCING METHOD FOR SINGLE NUCLEIC ACID MOLECULE

Abstract

A label-free sequencing method for a single molecular nucleic acid is provided. The primer is paired with the nucleic acid template to be assembled to a polymerase. When the nucleotides are added, the electrical conductance signal is measured by the polymerase being connected to the protein transistor to determine the sequences of the nucleic acid template. The trajectory of the measured electrical conductance signal contains plateaux with obvious spikes, which is used to identify four types of the nucleotides and their bases. Furthermore, the sequencing method is suitable for sequencing of complex nucleic acids.