Metabolic variation of HeLa cells migrating on microfabricated cytophilic channels studied by the fluorescence lifetime of NADH

Title:	Metabolic variation of HeLa cells migrating on microfabricated cytophilic channels studied by the fluorescence lifetime of NADH
Authors:	Deka, Gitanjal Okano, Kazunori Masuhara, Hiroshi Li, Yaw-Kuen Kao, Fu-Jen 應用化學系應用化學系分子科學碩博班光電工程學系 Department of Applied Chemistry Institute of Molecular science Department of Photonics
Issue Date:	1-Jan-2014
Abstract:	We report a novel method for studying cellular migration in vitro. Cytophilic microdomains were formed on a cytophobic substrate by laser ablation. HeLa cells were grown on those domains until confluence, and then channels were formed to guide cellular migration. Two-photon excitation fluorescence-lifetime imaging of NADH revealed metabolic variation among migrating and nonmigrating cells.
URI:	http://dx.doi.org/10.1039/c4ra06492e http://hdl.handle.net/11536/124003
ISSN:	2046-2069
DOI:	10.1039/c4ra06492e
Journal:	RSC ADVANCES
Issue:	83
Begin Page:	44100
End Page:	44104
Appears in Collections:	Articles

Files in This Item:

If it is a zip file, please download the file and unzip it, then open index.html in a browser to view the full text content.