以密閉式藻類毒性詴驗方法評估反應性與麻醉性有機物之混合毒性研究

Abstract

本研究以連續式培養批次式試驗來針對氯酚類與醛類進行密閉式藻類混合毒性試驗藉以評估混合毒性效應，並利用本研究群所採用的混合預測模式來進行預測與比較，更進一步的利用Isobologram針對不符合的試驗加以討論。 進行混合毒性試驗時，以ΔDO之EC50值為依據進行1：1的毒性單位比之混合毒性試驗，各個反應終點EC50的不同Final yield及Growth rate的毒性單位比則不為1：1。實驗結果發現，在ΔDO此反應終點之15組混合毒性試驗有11組符合模式預測，符合比例高達73.3%；而在Final yield及Growth rate由於其毒性單位比相差過大，以致於與模式較不符合。但在此兩反應終點由於其相對斜率較大，因此出現拮抗或相加的機率有大幅提升的現象，顯示本研究所考慮的單一劑量反應曲線的斜率之重要性。此外，在物種比較之下亦發現月芽藻的預測能力來的比Microtox高出許多。 針對不符合的試驗進行HPLC的分析，可發現到3-Hydroxybenzaldehyd -e在1.3秒時有出現新的物質，在與個別單一毒物進行分析的結果比較發現，當含有營養基值時會有此一反應，無營養基值則否，再經過一些試驗結果發現此為一耗氧反應；交互作用主要討論兩毒性化學物質間的作用，但在分析結果發現並非如此，因此可預想其為更複雜的情況以致於實驗結果與模式不符合，使的本研究所評估之毒性化學物質的混合毒性效應時會更為複雜。

This study is using a close-system algae toxic tests to evaluate the toxicity of chlorophenols and aldehydes combined effects. By using RA model to make predictions and comparison. Furthermore, using the Isobologram discusses the data which do not tally anticipated . Mixture toxicity tests using 1:1 mixture of toxic units which are according to the ΔDO and the EC50 value. Because the end point of EC50 was different to Final yield and Growth rate, so the toxic units was not 1:1 for both of them. The results found out that there are11 tests fit the model predictions from 15 mixture toxicity tests which is base on the end point of ΔDO ,and the proportion was as high as 73.3%;but toxic units of final yield and growth rate differ too large, can not follow the predictions . However, because the slope of final yield and growth rate are relatively large, it enhanced the chances of antagonistic or additive. In addition, comparison of species showed that R.subcapitata had a higher predictive abilityr than Microtox. For the unfitting tests, by using HPLC analysis found out that 3-Hydroxybenzaldehyd-e at 1.3 seconds emergences new substances. Comparing with single individual comparison found that when the base contains the nutritional value would show this one response, non-nutrient-based values were not. After some testing and found this as an oxygen reaction. This study should focused on the interaction between two chemical substances iii toxic effect, but in this case the result of HPLC analysis was not follow. so that in this study assessed the toxic mix of chemical substances toxic effects will be more complex.