標題: | Automatic method to compare the lanes in gel electrophoresis images |
作者: | Lin, Chih-Yang Ching, Yu-Tai Yang, Yun-Liang 生物科技學系 資訊工程學系 Department of Biological Science and Technology Department of Computer Science |
關鍵字: | dynamic programming;gel electrophoresis (GE);lane comparison;matched filter;segmentation;watershed method |
公開日期: | 1-三月-2007 |
摘要: | Gel electrophoresis (GE) is an important tool in genomic analysis. GE results are presented using images. Each image contains several vertical lanes. Each lane consists of several horizontal bands. Two lanes are identical if the relative positions of the bands are the same. We present a computer method designed to compare the lanes and identify identical lanes. This method, developed using many image-processing techniques, is applied to segment the lanes and bands in GE images. The lanes are then converted into "position vectors" that describe the positions of the bands. Comparing lanes becomes equivalent to comparing the position vectors. This method can accurately identify identical lanes, helping biologists to identify the identical lanes from many lanes with much less effort. |
URI: | http://dx.doi.org/10.1109/TITB.2006.875661 http://hdl.handle.net/11536/11094 |
ISSN: | 1089-7771 |
DOI: | 10.1109/TITB.2006.875661 |
期刊: | IEEE TRANSACTIONS ON INFORMATION TECHNOLOGY IN BIOMEDICINE |
Volume: | 11 |
Issue: | 2 |
起始頁: | 179 |
結束頁: | 189 |
顯示於類別: | 期刊論文 |