標題: YY Ubiquitin-coated nanodiamonds bind to autophagy receptors for entry into the selective autophagy pathway
作者: Liu, Kuang-Kai
Qiu, Wei-Ru
Raj, Emmanuel Naveen
Liu, Huei-Fang
Huang, Hou-Syun
Lin, Yu-Wei
Chang, Chien-Jen
Chen, Ting-Hua
Chen, Chinpiao
Chang, Huan-Cheng
Hwang, Jenn-Kang
Chao, Jui-I
生物科技學系
生物資訊及系統生物研究所
分子醫學與生物工程研究所
Department of Biological Science and Technology
Institude of Bioinformatics and Systems Biology
Institute of Molecular Medicine and Bioengineering
關鍵字: autophagy receptors;lysosome;nanodiamonds;selective autophagy;ubiquitin
公開日期: 2017
摘要: Selective macroautophagy/autophagy plays a pivotal role in the processing of foreign pathogens and cellular components to maintain homeostasis in human cells. To date, numerous studies have demonstrated the uptake of nanoparticles by cells, but their intracellular processing through selective autophagy remains unclear. Here we show that carbon-based nanodiamonds (NDs) coated with ubiquitin (Ub) bind to autophagy receptors (SQSTM1 [sequestosome 1], OPTN [optineurin], and CALCOCO2/NDP52 [calcium binding and coiled-coil domain 2]) and are then linked to MAP1LC3/LC3 (microtubule-associated protein 1 light chain 3) for entry into the selective autophagy pathway. NDs are ultimately delivered to lysosomes. Ectopically expressed SQSTM1-green fluorescence protein (GFP) could bind to the Ub-coated NDs. By contrast, the Ub-associated domain mutant of SQSTM1 (UBA)-GFP did not bind to the Ub-coated NDs. Chloroquine, an autophagy inhibitor, prevented the ND-containing autophagosomes from fusing with lysosomes. Furthermore, autophagy receptors OPTN and CALCOCO2/NDP52, involved in the processing of bacteria, were found to be involved in the selective autophagy of NDs. However, ND particles located in the lysosomes of cells did not induce mitotic blockage, senescence, or cell death. Single ND clusters in the lysosomes of cells were observed in the xenografted human lung tumors of nude mice. This study demonstrated for the first time that Ub-coated nanoparticles bind to autophagy receptors for entry into the selective autophagy pathway, facilitating their delivery to lysosomes.
URI: http://dx.doi.org/10.1080/15548627.2016.1254864
http://hdl.handle.net/11536/133320
ISSN: 1554-8627
DOI: 10.1080/15548627.2016.1254864
期刊: AUTOPHAGY
Volume: 13
Issue: 1
起始頁: 187
結束頁: 200
顯示於類別:期刊論文