標題: | Surface Cleaning of the Nanowire Field-Effect Transistor for Gene Detection |
作者: | Wu, Chi-Chang Liu, Fu-Ken Lin, Ling-Hung Pang, See-Tong Chuang, Cheng-Keng Pan, Tung-Ming Ou, Keng-Liang Ko, Fu-Hsiang 材料科學與工程學系 Department of Materials Science and Engineering |
關鍵字: | Nanowire;Biosensor;Surface Cleaning;DNA |
公開日期: | 1-十二月-2011 |
摘要: | The efficiency of DNA immobilization by using various surface cleaning methods is studied in this work. The degree of surface cleaning is evaluated by the surface tension measurement to reveal the contribution from the polar and apolar terms. The observation from the fluorescent microscope images indicates the effectiveness of surface clean by the acetone and ethanol mixtures, as well as the sulphuric acid and hydrogen peroxide mixtures. We also fabricate a series of back-gated, 60-nm nanowired (NW) field-effect transistor (FET) sensors for mutation gene detection by following the developed acetone and ethanol mixtures. Electrical properties of the NWFET sensor demonstrate the n-channel depletion characteristics. The current of the sensor is reduced once the attachment of negative charge molecules. The single-stranded capture DNA is chemically immobilized onto the surface of silicon NWFET by three-step reactions. The sensor surface demonstrates the great performance of current shift after the suitable cleaning. The NWFET sensor is successfully applied to detect the BRAF(V599E) mutation genes from the hybridized processes. The sensing behaviour estimated from the electrical signal reaches the femtomolar level. |
URI: | http://dx.doi.org/10.1166/jnn.2011.3952 http://hdl.handle.net/11536/15087 |
ISSN: | 1533-4880 |
DOI: | 10.1166/jnn.2011.3952 |
期刊: | JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY |
Volume: | 11 |
Issue: | 12 |
起始頁: | 10639 |
結束頁: | 10643 |
顯示於類別: | 會議論文 |