標題: Photothermal Microscopy for High Sensitivity and High Resolution Absorption Contrast Imaging of Biological Tissues
作者: Miyazaki, Jun
Kobayahsi, Takayoshi
電子物理學系
Department of Electrophysics
關鍵字: photothermal effect;pump-probe microscopy;superresolution
公開日期: 1-六月-2017
摘要: Photothermal microscopy is useful to visualize the distribution of non-fluorescence chromoproteins in biological specimens. Here, we developed a high sensitivity and high resolution photothermal microscopy with low-cost and compact laser diodes as light sources. A new detection scheme for improving signal to noise ratio more than 4-fold is presented. It is demonstrated that spatial resolution in photothermal microscopy is up to nearly twice as high as that in the conventional widefield microscopy. Furthermore, we demonstrated the ability for distinguishing or identifying biological molecules with simultaneous muti-wavelength imaging. Simultaneous photothermal and fluorescence imaging of mouse brain tissue was conducted to visualize both neurons expressing yellow fluorescent protein and endogenous non-fluorescent chromophores.
URI: http://dx.doi.org/10.3390/photonics4020032
http://hdl.handle.net/11536/153570
DOI: 10.3390/photonics4020032
期刊: PHOTONICS
Volume: 4
Issue: 2
起始頁: 0
結束頁: 0
顯示於類別:期刊論文