標題: | Photothermal Microscopy for High Sensitivity and High Resolution Absorption Contrast Imaging of Biological Tissues |
作者: | Miyazaki, Jun Kobayahsi, Takayoshi 電子物理學系 Department of Electrophysics |
關鍵字: | photothermal effect;pump-probe microscopy;superresolution |
公開日期: | 1-六月-2017 |
摘要: | Photothermal microscopy is useful to visualize the distribution of non-fluorescence chromoproteins in biological specimens. Here, we developed a high sensitivity and high resolution photothermal microscopy with low-cost and compact laser diodes as light sources. A new detection scheme for improving signal to noise ratio more than 4-fold is presented. It is demonstrated that spatial resolution in photothermal microscopy is up to nearly twice as high as that in the conventional widefield microscopy. Furthermore, we demonstrated the ability for distinguishing or identifying biological molecules with simultaneous muti-wavelength imaging. Simultaneous photothermal and fluorescence imaging of mouse brain tissue was conducted to visualize both neurons expressing yellow fluorescent protein and endogenous non-fluorescent chromophores. |
URI: | http://dx.doi.org/10.3390/photonics4020032 http://hdl.handle.net/11536/153570 |
DOI: | 10.3390/photonics4020032 |
期刊: | PHOTONICS |
Volume: | 4 |
Issue: | 2 |
起始頁: | 0 |
結束頁: | 0 |
顯示於類別: | 期刊論文 |