標題: Clusters of Circulating let-7 Family Tumor Suppressors Are Associated with Clinical Characteristics of Chronic Hepatitis C
作者: Tsai, Yi-Shan
Yeh, Ming-Lun
Tsai, Pei-Chien
Huang, Ching-, I
Huang, Chung-Feng
Hsieh, Meng-Hsuan
Liu, Ta-Wei
Lin, Yi-Hung
Liang, Po-Cheng
Lin, Zu-Yau
Chen, Shinn-Cherng
Huang, Jee-Fu
Chuang, Wan-Long
Dai, Chia-Yen
Yu, Ming-Lung
生醫工程研究所
Institute of Biomedical Engineering
關鍵字: chronic hepatitis C;let-7 family;hepatitis C virus;miRNA;biomarker;hepatocellular carcinoma
公開日期: 1-七月-2020
摘要: Hepatitis C virus (HCV) infections can cause permanent liver-related diseases, including hepatocellular carcinoma (HCC). Low mortality and incidence of HCC have been observed in patients with chronic hepatitis C undergoing direct-acting antiviral therapy. Tumor suppressive let-7 family members are down-regulated in HCC. The present study, therefore, aimed to investigate whether expression levels for the full spectrum of let-7 family members (let-7a, 7b, 7c, 7d, 7e, 7f, 7g, 7i, and miR-98) in the circulatory system are useful as surveillance biomarkers for liver-related diseases to monitor treatment efficacy during HCV infection. To this end, we measured the levels of mature circulating let-7 family members using quantitative reverse transcription-PCR in 236 patients with HCV infection, and 147 age- and sex-matched controls. Using hierarchical cluster analysis and principal component analysis, three clusters were obtained after measuring expression levels of let-7 family members in the patients and controls. Cluster 1 included let-7a/d/e/g, Cluster 2 comprised let-7b and let-7i, and Cluster 3 comprised let-7c/f/miR-98. Let-7b/c/g represented the three clusters and showed the best survival response to liver cancer when analyzed with respect to patient data. Therefore, considering the circulating levels of let7 b/c/g as representatives of the let-7 family may facilitate effective monitoring of liver-related disease.
URI: http://dx.doi.org/10.3390/ijms21144945
http://hdl.handle.net/11536/155108
DOI: 10.3390/ijms21144945
期刊: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume: 21
Issue: 14
起始頁: 0
結束頁: 0
顯示於類別:期刊論文