On the origin of the 1602 cm-1 Raman band of yeasts; contribution of ergosterol

Abstract

The 1602 cm1 Raman signature, which we call the Raman spectroscopic signature of life in yeasts, is a marker Raman band for cell metabolic activity. Despite the established fact that its intensity sensitively reflects the metabolic status of the cell, its molecular origin remained unclear. In this work, we propose ergosterol as the major contributor of the 1602 cm1 Raman signature. The theoretical isotope shift calculation for ergosterol agreed with previous observations. Furthermore, experiments showed that the Raman spectrum of ergosterol corresponds very well with the depleting spectral component in yeast that behaves together with the 1602 cm1 signature when the cells are under stress. This work implies that the 1602 cm1 Raman signature could serve as an intrinsic ergosterol marker in yeasts for the study of sterol metabolism in vivo and in a label-free manner, which could not be done by any other techniques at the current stage. ((c) 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)