標題: | Reactant Minimization during Sample Preparation on Digital Microfluidic Biochips using Skewed Mixing Trees |
作者: | Huang, Juinn-Dar Liu, Chia-Hung Chiang, Ting-Wei 電子工程學系及電子研究所 Department of Electronics Engineering and Institute of Electronics |
關鍵字: | Biochip;digital microfluidic biochip (DMFB);dilution;sample preparation;reactant minimization;mixing tree |
公開日期: | 2012 |
摘要: | Sample preparation is an indispensable process to biochemical reactions. Original reactants are usually diluted to the solutions with desirable concentrations. Since the reactants, like infant's blood, DNA evidence collected from a crime scene, or costly reagents, are extremely valuable, the usage of reactant must be minimized in the sample preparation process. In this paper, we propose the first reactant minimization approach, REMIA, during sample preparation on digital microfluidic biochips (DMFBs). Given a target concentration, REMIA constructs a skewed mixing tree to guide the sample preparation process for reactant minimization. Experimental results demonstrate that REMIA can save about 31%similar to 52% of reactant usage on average compared with three existing sample preparation methods. Besides, REMIA can be extended to tackle the sample preparation problem with multiple target concentrations, and the extended version also successfully decreases the reactant usage further. |
URI: | http://hdl.handle.net/11536/21595 |
ISBN: | 978-1-4503-1573-9 |
期刊: | 2012 IEEE/ACM INTERNATIONAL CONFERENCE ON COMPUTER-AIDED DESIGN (ICCAD) |
起始頁: | 377 |
結束頁: | 383 |
顯示於類別: | 會議論文 |