標題: | Hypochlorous acid turn-on boron dipyrromethene probe based on oxidation of methyl phenyl sulfide |
作者: | Liu, Shi-Rong Vedamalai, Mani Wu, Shu-Pao 應用化學系 Department of Applied Chemistry |
關鍵字: | Hypochlorous acid;Fluorescent probe;Boron dipyrromethene;Bioimaging |
公開日期: | 24-十月-2013 |
摘要: | A boron dipyrromethene (BODIPY)-based fluorometric probe, HCS, has been successfully developed for the highly sensitive and selective detection of hypochlorous acid (HOCl). The probe is based on the specific HOCl-promoted oxidation of methyl phenyl sulfide. The reaction is accompanied by a 160-fold increase in the fluorescent quantum yield (from 0.003 to 0.480). The fluorescent turn-on mechanism is accomplished by suppression of photoinduced electron transfer (PET) from the methyl phenyl sulfide group to BODIPY. The fluorescence intensity of the reaction between HOCl and HCS shows a good linearity in the HOCl concentration range 1-10 mu M. The detection limit is 23.7 nM (S/N = 3). In addition, confocal fluorescence microscopy imaging using RAW264.7 macrophages demonstrates that the HCS probe could be an efficient fluorescent detector for HOCl in living cells. (C) 2013 Published by Elsevier B.V. |
URI: | http://dx.doi.org/10.1016/j.aca.2013.09.018 http://hdl.handle.net/11536/22969 |
ISSN: | 0003-2670 |
DOI: | 10.1016/j.aca.2013.09.018 |
期刊: | ANALYTICA CHIMICA ACTA |
Volume: | 800 |
Issue: | |
起始頁: | 71 |
結束頁: | 76 |
顯示於類別: | 期刊論文 |