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dc.contributor.authorYeh, Fang-Yuanen_US
dc.contributor.authorTseng, I-Huaen_US
dc.contributor.authorChuang, Shu-Hungen_US
dc.contributor.authorLin, Chih-Shengen_US
dc.date.accessioned2014-12-08T15:36:15Z-
dc.date.available2014-12-08T15:36:15Z-
dc.date.issued2014en_US
dc.identifier.issn2046-2069en_US
dc.identifier.urihttp://hdl.handle.net/11536/24585-
dc.identifier.urihttp://dx.doi.org/10.1039/c4ra00258jen_US
dc.description.abstractPancreatitis is the inflammation of the pancreas. Chymotrypsin, an indicator of pancreatic function, could serve as a biomarker for the diagnosis of pancreatitis. A gold nanoparticle (AuNP)-based fluorescence assay was fabricated in this study to assay the activity of chymotrypsin. Peptides labeled with fluorophores were conjugated onto AuNPs as a chymotrypsin-activated peptide-functionalized AuNP probe (AuNPs-peptide probe). The detection sensitivity of the AuNPs-peptide probe toward proteolytic activity was significantly increased by using spacer-enhanced peptides with specifically designed lengths and charges. The limit of detection of the designed AuNPs-peptide probe decreased, enabling detection at the pM level within a 15 min detection time. The AuNPs-peptide probe was used to evaluate chymotrypsin activity as an indicator of acute pancreatitis (AP) in a mouse model induced by cerulein challenge. The fecal chymotrypsin activity in cerulein-induced AP mice was significantly lower than that observed in controls. This is the first study to use an AuNPs-peptide probe for the diagnosis of pancreatitis using fecal specimens.en_US
dc.language.isoen_USen_US
dc.titleSpacer-enhanced chymotrypsin-activated peptide-functionalized gold nanoparticle probes: a rapid assay for the diagnosis of pancreatitisen_US
dc.typeArticleen_US
dc.identifier.doi10.1039/c4ra00258jen_US
dc.identifier.journalRSC ADVANCESen_US
dc.citation.volume4en_US
dc.citation.issue42en_US
dc.citation.spage22266en_US
dc.citation.epage22276en_US
dc.contributor.department生物科技學系zh_TW
dc.contributor.departmentDepartment of Biological Science and Technologyen_US
dc.identifier.wosnumberWOS:000337123100068-
dc.citation.woscount1-
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