標題: | DIFFERENTIATION OF NEURAL STEM/PROGENITOR CELLS USING LOW-INTENSITY ULTRASOUND |
作者: | Lee, I-Chi Lo, Tsu-Lin Young, Tai-Horng Li, Yi-Chen Chen, Nelson G. Chen, Chung-Hsuan Chang, Ying-Chih 交大名義發表 電機資訊學士班 National Chiao Tung University Undergraduate Honors Program of Electrical Engineering and Computer Science |
關鍵字: | Low-intensity ultrasound;Neural stem/progenitor cells;Neurite outgrowth;Neuron;Induction;Neuron network |
公開日期: | 1-九月-2014 |
摘要: | Herein, we report the evaluation of apoptosis, cell differentiation, neurite outgrowth and differentiation of neural stem/progenitor cells (NSPCs) in response to low-intensity ultrasound (LIUS) exposure. NSPCs were cultured under different conditions, with and without LIUS exposure, to evaluate the single and complex effects of LIUS. A lactic dehydrogenase assay revealed that the cell viability of NSPCs was maintained with LIUS exposure at an intensity range from 100 to 500 mW/cm(2). Additionally, in comparison with no LIUS exposure, the cell survival rate was improved with the combination of medium supplemented with nerve growth factor and LIUS exposure. Our results indicate that LIUS exposure promoted NSPC attachment and differentiation on a glass substrate. Neurite outgrowth assays revealed the generation of longer, thicker neurites after LIUS exposure. Furthermore, LIUS stimulation substantially increased the percentage of differentiating neural cells in NSPCs treated with nerve growth factor in comparison with the unstimulated group. The high percentage of differentiated neural cells indicated that LIUS induced neuronal networks denser than those observed in the unstimulated groups. Furthermore, the release of nitric oxide, an important small-molecule neurotransmitter, was significantly upregulated after LIUS exposure. It is therefore reasonable to suggest that LIUS promotes the differentiation of NSPCs into neural cells, induces neurite outgrowth and regulates nitric oxide production; thus, LIUS may be a potential candidate for NSPC induction and neural cell therapy. (E-mail: iclee@mail.cgu.edu.tw or yingchih@gate.sinica.edu.tw) (C) 2014 World Federation for Ultrasound in Medicine & Biology. |
URI: | http://dx.doi.org/10.1016/j.ultrasmedbio.2014.05.001 http://hdl.handle.net/11536/25215 |
ISSN: | 0301-5629 |
DOI: | 10.1016/j.ultrasmedbio.2014.05.001 |
期刊: | ULTRASOUND IN MEDICINE AND BIOLOGY |
Volume: | 40 |
Issue: | 9 |
起始頁: | 2195 |
結束頁: | 2206 |
顯示於類別: | 期刊論文 |