標題: DIFFERENTIATION OF NEURAL STEM/PROGENITOR CELLS USING LOW-INTENSITY ULTRASOUND
作者: Lee, I-Chi
Lo, Tsu-Lin
Young, Tai-Horng
Li, Yi-Chen
Chen, Nelson G.
Chen, Chung-Hsuan
Chang, Ying-Chih
交大名義發表
電機資訊學士班
National Chiao Tung University
Undergraduate Honors Program of Electrical Engineering and Computer Science
關鍵字: Low-intensity ultrasound;Neural stem/progenitor cells;Neurite outgrowth;Neuron;Induction;Neuron network
公開日期: 1-九月-2014
摘要: Herein, we report the evaluation of apoptosis, cell differentiation, neurite outgrowth and differentiation of neural stem/progenitor cells (NSPCs) in response to low-intensity ultrasound (LIUS) exposure. NSPCs were cultured under different conditions, with and without LIUS exposure, to evaluate the single and complex effects of LIUS. A lactic dehydrogenase assay revealed that the cell viability of NSPCs was maintained with LIUS exposure at an intensity range from 100 to 500 mW/cm(2). Additionally, in comparison with no LIUS exposure, the cell survival rate was improved with the combination of medium supplemented with nerve growth factor and LIUS exposure. Our results indicate that LIUS exposure promoted NSPC attachment and differentiation on a glass substrate. Neurite outgrowth assays revealed the generation of longer, thicker neurites after LIUS exposure. Furthermore, LIUS stimulation substantially increased the percentage of differentiating neural cells in NSPCs treated with nerve growth factor in comparison with the unstimulated group. The high percentage of differentiated neural cells indicated that LIUS induced neuronal networks denser than those observed in the unstimulated groups. Furthermore, the release of nitric oxide, an important small-molecule neurotransmitter, was significantly upregulated after LIUS exposure. It is therefore reasonable to suggest that LIUS promotes the differentiation of NSPCs into neural cells, induces neurite outgrowth and regulates nitric oxide production; thus, LIUS may be a potential candidate for NSPC induction and neural cell therapy. (E-mail: iclee@mail.cgu.edu.tw or yingchih@gate.sinica.edu.tw) (C) 2014 World Federation for Ultrasound in Medicine & Biology.
URI: http://dx.doi.org/10.1016/j.ultrasmedbio.2014.05.001
http://hdl.handle.net/11536/25215
ISSN: 0301-5629
DOI: 10.1016/j.ultrasmedbio.2014.05.001
期刊: ULTRASOUND IN MEDICINE AND BIOLOGY
Volume: 40
Issue: 9
起始頁: 2195
結束頁: 2206
顯示於類別:期刊論文