四個二價金屬錯合物單經結構之決定與蛋白質養晶

Abstract

本論文分為兩部份，第一部份為利用X光繞射法來決定四個二價金屬配位 錯合物的單晶結構；第二部份為眼鏡蛇毒蛋白Ⅳ的養晶方法及過程。第一 部份：清華大學化學系鍾崇燊教授所提供的四個晶體，由成大地科系董淑 芳小姐收集X光繞射資料，吾人利用X光繞射數據來決定其單晶結構，此四 個晶體分別為以銅為中心的扭曲雙三角錐型五配位錯合物；以銅為中心的 扭曲雙金字塔型六配位錯合物；以鎳為中心的扭曲雙金字塔型六配位錯合 物及以鎳為中心的四配位錯合物。第二部份：清華大學生科系楊振忠院士 所提供的眼鏡蛇毒蛋白Ⅳ，吾人利用蒸汽擴散法中的懸滴法來養晶，所使 用的養晶環境為文獻中的48種條件，嘗試在此48種條件中找出最適合眼鏡 蛇毒蛋白Ⅳ的養晶條件。 This paper includes two parts. One is to determine the structure of the single crystal of four Metal(Ⅱ) Complexes by X-ray diffraction. The other is the method and process in the crystal growth for the protein of CardiotoxinⅣ First: The four crystals were provided by Prof. Chung-Sun Chung in the Chemistry Department of NTHU. We determined the structure of the 4 single crystals by X-ray diffraction. Of the four complexes, copper(Ⅱ) ion is five coordinated with geometry of distorted trigonal bipyramid structure in the first crystal. Copper(Ⅱ) ion has six coordinations with distorted square bipyramid in the second crystal. In the third, nickel(Ⅱ) ion has six coordinations with four N atoms equatorial and N atoms of the isothiocyanate groups axial, the same geometry as the second crystal. Nickel(Ⅱ) ion is four-coordinate with four nitrogen atoms in the macrocyclic plane in the last crystal.Second: The purified material of CardiotoxinⅣwas provided by Prof. C.C. Yang in the Department of Life Science of NTHU. We used hanging drop of vapor diffusion method to grow protein of crystal. We tried to screen the best condition for the crystallization of CadiotoxinⅣ from 48 conditions.