Browsing by Author "Baruzzi, Federico"

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Direct Plasma Deposition of Lysozyme-Embedded Bio-Composite Thin Films
(2015-11-01)
Palumbo, Fabio
;
Camporeale, Giuseppe
;
Yang, Yi-Wei
;
Wu, Jong-Shinn
;
Sardella, Eloisa
Bio-composite coatings, consisting of an organic matrix embedding a bioactive molecule, have been deposited by means of atomizer-assisted atmospheric pressure plasma. Ethylene was chosen as the precursor of the matrix, while the atomizer was fed with a water solution of lysozyme. Coatings chemical composition was investigated by XPS, FTIR and MALDI-TOF spectroscopies, and it has been proved that the one-step inclusion of protein domains in the composite coatings is successful and lysozyme chemical structure is only slightly altered. The amount of embedded lysozyme is as high as 14 mg/cm(2) as evaluated from water release test. Finally, the activity of the plasma-embedded protein is close to that of pure lysozyme as verified against Micrococcus lysodeikticus ATCC 4698 through an agar plate diffusion test.
Direct Plasma Deposition of Lysozyme-Embedded Bio-Composite Thin Films
(2015-11)
Palumbo, Fabio
;
Camporeale, Giuseppe
;
Yang, Yi-Wei
;
Wu, Jong-Shinn
;
Sardella, Eloisa
Bio-composite coatings, consisting of an organic matrix embedding a bioactive molecule, have been deposited by means of atomizer-assisted atmospheric pressure plasma. Ethylene was chosen as the precursor of the matrix, while the atomizer was fed with a water solution of lysozyme. Coatings chemical composition was investigated by XPS, FTIR and MALDI-TOF spectroscopies, and it has been proved that the one-step inclusion of protein domains in the composite coatings is successful and lysozyme chemical structure is only slightly altered. The amount of embedded lysozyme is as high as 14 mg/cm(2) as evaluated from water release test. Finally, the activity of the plasma-embedded protein is close to that of pure lysozyme as verified against Micrococcus lysodeikticus ATCC 4698 through an agar plate diffusion test.