Browsing by Author "Campagnola, Paul J."

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Fast and improved bioimaging via temporal focusing multiphoton excitation microscopy with binary digital-micromirror-device holography
(2018-11-01)
Sie, Yong Da
;
Chang, Chia-Yuan
;
Lin, Chun-Yu
;
Chang, Nan-Shan
;
Campagnola, Paul J.
Conventional temporal focusing-based multiphoton excitation microscopy (TFMPEM) can offer wide-field optical sectioning with an axial excitation confinement of a few microns. To improve the axial confinement of TFMPEM, a binary computer-generated Fourier hologram (CGFH) via a digital-micromirror-device (DMD) was implemented to intrinsically improve the axial confinement by filling the back-focal aperture of the objective lens. Experimental results show that the excitation focal volume can be condensed and the axial confinement improved about 24% according to the DMD holography. In addition, pseudouniform MPE can be achieved using two complementary CGFHs with rapid pulse-width modulation switching via the DMD. Furthermore, bioimaging of CV-1 in origin with SV40 genes-7 cells demonstrates that the TFMPEM with binary DMD holography can improve image quality by enhancing axial excitation confinement and rejecting out-of-focus excitation. (C) 2018 Society of Photo-Optical Instrumentation Engineers (SPIE)
Graphene oxide dot-based microstructures via dispersion and support of bovine serum albumin
(2016-10-01)
Chang, Hsin-Yu
;
Lin, Chun-Yu
;
Chang, Chia-Yuan
;
Teng, Hsisheng
;
Campagnola, Paul J.
Three-dimensional graphene oxide-based microstructures have great potential for use in microelectronic and biomedical applications and can be fabricated by using two-photon excitation photochemical processes. In such excitation processes, rose Bengal is the preferred candidate as the photoactivator in two-photon crosslinking; nevertheless, it induces graphene oxide self-aggregation. Herein, bovine serum albumin (BSA) is adopted to prevent graphene oxide dot (GOD) aggregation even at very high GOD fabrication-solution concentrations, yet still supports GOD microstructures. In this manner, three-dimensional freeform GOD microstructures with different GOD concentrations were developed. Furthermore, the biocomparability of the GOD microstructure with the help of added biomaterials such as BSA was verified under living cell incubation. (C) 2016 Optical Society of America