Browsing by Author "Chan, Chia-Hao"

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Immobilization of layered double hydroxides in the fluidic system for nanoextraction of specific DNA molecules
(2008-02-04)
Chen, Jem-Kun
;
Chan, Chia-Hao
;
Chang, Feng-Chih
;
應用化學系
;
Department of Applied Chemistry
The purpose of this study was to immobilize inorganic layered double hydroxides (LDHs) on the poly(methylmethacrylate) substrate as the media to extract the specific DNA molecules through fluidic system to enhance the efficiency of extract specific DNA molecules from extremely low concentration in sample solution. LDH immobilized through solvent swelling and plasma treatment on the polymer surface captured the specific DNA molecules lysed from Escherichia coli (E. coli) cells as the target DNA molecules with 2x10(-4) g/l of concentration in sample solution mixed biomacromolecules lysed from human blood. The encapsulated DNA molecules released through dissolving of LDHs by slight acid (pH=4-5) solution then amplified by polymerase chain reaction (PCR) process through the primers for E. coli cells. The DNA molecules amplified by PCR process were characterized by gel electrophoresis to recognize the existence of E. coli cells. The results show that immobilized LDHs could be regarded as the specific DNA detector for rapid disease diagnosis through fluidic system. (C) 2008 American Institute of Physics.
Immobilization of Layered double Hydroxides in the Fluidic System for Nanoextraction of Specific DNA Molecules
(2010-01-01)
Chen, Jem-Kun
;
Chan, Chia-Hao
;
Chang, Feng-Chih
;
應用化學系
;
Department of Applied Chemistry
The purpose of this study was to immobilize inorganic layered double hydroxides (LDHs) on the poly(methylmethacrylate) (PMMA) substrate as the media to extract the specific DNA molecules through fluidic system to enhance the efficiency of extract specific DNA molecules from extreme low concentration in sample solution. The encapsulated DNA molecules released through dissolve of LDHs by slight acid (PH=4-5) solution then amplified by polymerase chain reaction (PCR), process through the primers for E. coli cells. The results show that immobilized LHDs could be regarded as the specific DNA detector in the sample solution mixed biomacromolecules for rapid disease diagnosis through fluidic system.
Remote Magnetic Control of Autophagy in Mouse B-Lymphoma Cells with Iron Oxide Nanoparticles
(2019-04-01)
Lin, You-Rong
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Chan, Chia-Hao
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Lee, Hui-Ting
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Cheng, Sheng-Jen
;
Yang, Jia-Wei
Autophagy is the spontaneous degradation of intracellular proteins and organelles in response to nutrient deprivation. The phagocytosis of iron oxide nanoparticles (IONPs) results in intracellular degradation that can be exploited for use in cancer treatment. Non-invasive magnetic control has emerged as an important technology, with breakthroughs achieved in areas such as magneto-thermal therapy and drug delivery. This study aimed to regulate autophagy in mouse B-lymphoma cells (A20) through the incorporation of IONPs-quantum dots (QDs). We hypothesized that with the application of an external magnetic field after phagocytosis of IONPs-QDs, autophagy of intracellular IONPs-QDs could be regulated in a non-invasive manner and subsequently modulate the regulation of inflammatory responses. The potential of this approach as a cancer treatment method was explored. The application of IONPs and an external magnetic force enabled the non-invasive regulation of cell autophagy and modulation of the self-regulatory function of cells. The combination of non-invasive magnetic fields and nanotechnology could provide a new approach to cancer treatment.
Specific DNA extraction through fluid channels with immobilization of layered double hydroxides on polycarbonate surface
(2008-07-28)
Chan, Chia-Hao
;
Chen, Jem-Kun
;
Chang, Feng-Chih
;
應用化學系
;
Department of Applied Chemistry
The purpose of this study was to immobilize inorganic layered double hydroxides (LDHs) on the polycarbonate (PC) substrate as the media to extract the specific DNA molecules through fluidic system and to enhance the extraction efficiency of specific DNA molecules from extreme low concentration in sample solution. LDH immobilized through solvent swelling and plasma treatment on the polymer surface captured the specific DNA molecules lysed from Escherichia coli (E. call) cells as the target DNA molecules with 2 x 10(-4) g/l of concentration in sample solution mixed biomacromolecules lysed from human blood. The encapsulated DNA molecules released through dissolve of LDHs by slight acid (pH 4-5) solution then amplified by polymerase chain reaction (PCR) process through the primers for E coli cells. The DNA molecules amplified by PCR process were characterized by gel electrophoresis to recognize the existence of E. coli cells. The results show that immobilized LDHs could be regarded as the specific DNA detector in the sample solution mixed biomacromolecules for rapid disease diagnosis through fluidic system to approach the lab-on-a-chip (LOC). (c) 2008 Elsevier B.V. All rights reserved.
TaN(x) thin films as copper barriers sputter-deposited at various NH(3)-to-Ar flow ratios
(2009-03-01)
Chen, Jem Kun
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Chan, Chia-Hao
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Kuo, Shiao-Wei
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Chang, Feng-Chih
;
應用化學系
To optimize the performance of copper diffusion barriers, we deposited TaN(x) thin films through radio frequency (RF) sputtering at various flow ratios of the reactive gases NH(3) and Ar. The composition of the film changed from Ta(2)N to TaN, as evidenced from deposition rates and N-to-Ta ratios, when we increased the NH(3)-to-Ar flow ratio from 0.075 to 0.3. Furthermore, the structure of the TaN(x) thin film transformed from body-centered cubic (BCC) to face-centered cubic (FCC) to nanocrystalline upon increasing the NH(3)-to-Ar flow rate, as revealed by the three steps in the rate of formation of the TaN(x) films during the sputtering process. When incorporated in Cu/TaN(x)/n(+) np(+) diodes, the thermal stability of the TaN(x) thin film-measured in terms of the leakage current remaining below 3 mu A-increased from 450 to 550 degrees C upon increasing the NH(3)-to-Ar flow ratio from 0.075 to 0.3. It appears that the NH(3)-to-Ar flow ratio influences the properties of TaN(x) films predominantly through modification of the crystal structure. (C) 2008 Published by Elsevier B.V.
TaN(x) thin films deposited through various flow ratios of N(2)/Ar for copper barrier properties
(2008)
Chen, Jem Kun
;
Chan, Chia-Hao
;
Chang, Feng-Chih
;
應用化學系
;
Department of Applied Chemistry
To optimize copper diffusion barriers, TaN(x) thin films for diffusion barriers were prepared by using radio frequency sputtering with various flow ratios of N(2)/Ar as the reactive gas. The component transformed from Ta(2)N to TaN as observed from deposition rates, and N/Ta ratios as N(2)/Ar flow ratios from 0.075 to 0.3. Furthermore, the structural transformations from body- centered cubic through face- centered cubic to nanocrystalline for TaN(x) thin film demonstrated under increase of N2/Ar flow rate, induced three- step stages for formation of TaN(x) films in the sputtering process. The thermal stabilities of the TaN(x) thin film in Cu/TaN(x)/n(+) np(+) diodes suggest that increasing flow ratio of N(2)/Ar from 0.075 to 0.3 enhanced the thermal stabilities from 450 to 550 degrees C under leakage current conditions below 3 mu A. The observation deduces that N2/Ar flow ratios dominated predominantly properties of TaN(x) films through crystal structure for barrier. c 2008 The Electrochemical Society.
Using imprinting technology to fabricate three-dimensional devices from moulds of thermosetting polymer patterns
(2006-09-01)
Chen, Jem-Kun
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Ko, Fu-Hsiang
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Chan, Chia-Hao
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Huang, Chih-Feng
;
Chang, Feng-Chih
The fabrication of moulds for imprinting can be simplified significantly by using specialized cross-linking polymers to define the pattern on a silicon wafer. Thermosetting polymers (SU-8) can be used to pattern silicon moulds for imprinting technologies because ( 1) silicon oxide moulds bearing a thermosetting polymer pattern can be obtained using conventional semiconductor technologies and ( 2) thermosetting polymers have no obvious glass transition temperature (T-g) because of their cross-linked structure, but the hardness decreases significantly when the temperature is above the Tg. In this study, we used Su-8 resist as the thermosetting polymer pattern to obtain moulds on a silicon wafer. We have tested the thermal properties of thermosetting (SU-8) and thermoplastic polymers (22A4) for use as imprinting patterns and imprinted resists. We fabricated a hill-like structure by applying an electron beam strategy and used this thick film to increase the adhesion between the pattern and the silicon wafer. We used scanning electron microscopy to investigate the resolution of the thermoplastic polymer resist ( 22A4) pattern that we imprinted using the thermosetting polymer (SU-8) pattern. To define the feature size after imprinting, we determined the feature size shrink factor after separation of the thermosetting polymer pattern (SU-8) from the thermoplastic polymer ( 22A4) resist. In addition, we have fabricated a microlens of polydimethylsiloxane ( PDMS) through replication using the thermoplastic polymer resist ( 22A4) obtained after imprinting the mould with the microlens structure of the thermosetting polymer ( SU- 8).