Browsing by Author "Chang, Chao-Yiu"

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A fluorescence turn-on probe for cysteine and homocysteine based on thiol-triggered benzothiazolidine ring formation
(2014-11-07)
Liu, Shi-Rong
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Chang, Chao-Yiu
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Wu, Shu-Pao
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應用化學系
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Department of Applied Chemistry
We synthesized a new coumarin-based probe TP, containing a disulfide moiety, to detect biothiols in cells. A fluorescence turn-on response is induced by the thiol-disulfide exchange of the probe, with subsequent intramolecular benzothiazolidine ring formation giving rise to a fluorescent product. The probe exhibits an excellent selectivity for cysteine (Cys) and homocysteine (Hcy) over glutathione (GSH) and other amino acids. The fluorescent probe also exhibits a highly sensitive fluorescence turn-on response to Cys and Hcy with detection limits of 0.8 mu M for Cys and 0.5 mu M for Hcy. In addition, confocal fluorescence microscopy imaging using RAW264.7 macrophages demonstrates that the probe TP could be an efficient fluorescent detector for thiols in living cells. (C) 2014 Elsevier B.V. All rights reserved.
Hydro gel light-guiding conjunction for absorptive type multi-ions detection
(2016-10-05)
Lin, Hung-Cheng
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Yang, Sean Tsungyeh
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Ng, Sock Hoon
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Zan, Hsiao-Wen
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Meng, Hsin-Fei
We present a novel miniaturized solid state sensor produced by embedding two optical fibers in a sensing hydro gel conjunction. The conjunction is a poly (2-hydroxyethyl methacrylate) (poly HEMA) hydro gel mixed with two kinds of selective fluorescent probes. Meso-2,6-Dichlorophenyltripyrrinone (TPN-Cl2) is used to react with zinc ion (Zn2+) and the synthesized "Chemosensor 1" is used to detect ferric ion (Fe3+). This hydro gel conjunction can serve as a light-guiding waveguide that allows light signals to transfer between two fibers. By using a multi-wavelength excitation light, a mixed solution that contains Zn2+ and Fe3+ can be detected in real-time. This miniaturized sensor develops an image for real-time monitoring of Zn2+ and Fe3+ ions in vivo. (C) 2016 Elsevier B.V. All rights reserved.