Browsing by Author "Chang, H. Y."

Now showing 1 - 4 of 4

A Novel Architecture to Build Ideal-linearity Neuromorphic Synapses on a Pure Logic FinFET Platform Featuring 2.5ns PGM-time and 10(12) Endurance
(2019-01-01)
Hsieh, E. R.
;
Chang, H. Y.
;
Chung, Steve S.
;
Chen, T. P.
;
Huang, S. A.
In this work, we will explore pure logic FinFET devices to realize the functionality of linear weight tuning capability as electric synapses. The unit cell of this new FinFET synapse is composed of two identical FinFETs in series; one serves as control and the other one as storage. This new FinFET synapse exhibits ideal linearity with nearly infinity training cycles (>10(12)). much lower programming voltage, 0.85V, and faster speed, 2.5ns. It can also analogically increase or decrease the transistor's V-th, to vary the drain conductance. As far as the analog performance is concerned, it performs excellent linearity and a wide tuning-window (20x) of weight-tuning capability. 1kb synaptic array has also been designed. The spice-simulated results have shown that new FinFET synaptic array can expand the array-size to 64x64, exhibiting 300x of SNR, w.r.t. that of RRAM array. Finally, the training of the neural network based on the proposed FinFET synapse can achieve 97.43% accuracy as high as the GPU one does.
High Efficiency E-coli Concentration via Dynamic Light-induced Optoelectro-osmosis Flow on Organic Photoconductivity Biochip
(2011)
Yang, S. M.
;
Lin, C. Y.
;
Wang, T. P.
;
Punde, T. H.
;
Chang, H. Y.
We demonstrate a dynamic light-induced optoelectro-osmosis flow design for high efficiency E. coli concentration on an organic photoconductive material based biochip. Several traditional approaches of bacteria purification and enrichment utilize magnetic beads coated with antibodies to attract them. The efficiency of collecting magnetic beads is equal to sort bacteria. Our work provides a direct method to enrich the E. coli concentration with dynamic light images. Due to the applications of photoconductive material, TiOPc, two features are demonstrated, simple biochip fabrication processes and rapid E. coli collection. The biochip is fabricated only with spin-coating a thin TiOPc layer, 500nm, on the indium tin oxide (ITO) conductive glass. Besides, the E. colt are concentrated together within 30 sec toward the illuminating region by the surface charge slip. We also calibrate the dominating effects in different applied AC frequency region. In conclusion, our light-induced E. coli manipulation approach would expand the scope of the examination efficiency, for example, water quality.
NUSAS: NEGATIVE PRESSURE DRIVING HEPG2/3T3 CELLS MIXING/GRADIENT CO-CULTURE INSIDE U TRAPPER ARRAY ON RAPID MULTICELLULAR SPHEROID ASSEMBLING SYSTEM
(2012)
Yang, S. M.
;
Fu, C. Y.
;
Tseng, S. Y.
;
Srinivasu, V. P.
;
Shilpa, S.
This article presents a Negative-pressure-powered U-trapper of Spheroid Assembling System (NUSAS) and four unique features are addressed: (1) Negative pressure powered medium driving, (2) HepG2/3T3 gradient distribution and mixing co-culture, (3) Cell trapping effect via gravity force and liquid flow co-activation, and (4) Large area of U trapper array. The development of in vitro liver device benefits liver toxicology and metabolism.[1,2] However, due to the complexity of human liver tissue, it has been rarely discussed what kind of hepatocyte to fibroblast ratio has nearest performance to a real liver tissue. Here, we culture liver/fibroblast cells with gradient ratio as multicellular assembling spheroids simultaneously to find this ratio. These results based on our efficient approaches give us meaningful foundations to investigate biomedical character of 3D HepG2/3T3 artificial tissue in different mixing cell conditions.
RmpA Regulation of Capsular Polysaccharide Biosynthesis in Klebsiella pneumoniae CG43
(2010-06-01)
Cheng, H. Y.
;
Chen, Y. S.
;
Wu, C. Y.
;
Chang, H. Y.
;
Lai, Y. C.
Sequence analysis of the large virulence plasmid pLVPK in Klebsiella pneumoniae CG43 revealed the presence of another mucoid factor encoding gene rmpA besides rmpA2. Promoter activity measurement indicated that the deletion of rmpA reduced K2 capsular polysaccharide (CPS) biosynthesis, resulting in decreased colony mucoidy and virulence in mice. Introduction of a multicopy plasmid carrying rmpA restored CPS production in the rmpA or rmpA2 mutant but not in the rcsB mutant. Transformation of the rmpA deletion mutant with an rcsB-carrying plasmid also failed to enhance CPS production, suggesting that a cooperation of RmpA with RcsB is required for regulatory activity. This was further corroborated by the demonstration of in vivo interaction between RmpA and RcsB using two-hybrid analysis and coimmunoprecipitation analysis. A putative Fur binding box was only found at the 5' noncoding region of rmpA. The promoter activity analysis indicated that the deletion of fur increased the rmpA promoter activity. Using electrophoretic mobility shift assay, we further demonstrated that Fur exerts its regulatory activity by binding directly to the promoter. As a result, the fur deletion mutant exhibited an increase in colony mucoidy, CPS production, and virulence in mice. In summary, our results suggested that RmpA activates CPS biosynthesis in K. pneumoniae CG43 via an RcsB-dependent manner. The expression of rmpA is regulated by the availability of iron and is negatively controlled by Fur.