Browsing by Author "Chang, Huan-Cheng"

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A facile ratiometric fluorescent chemodosimeter for hydrazine based on Ing-Manske hydrazinolysis and its applications in living cells
(2014-04-01)
Raju, Mandapati V. Ramakrishnam
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Prakash, Epperla Chandra
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Chang, Huan-Cheng
;
Lin, Hong-Cheu
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材料科學與工程學系
A facile and sensitive fluorescent probe for hydrazine was successfully constructed, displaying excellent colorimetric and ratiometric responses towards hydrazine via Ing-Manske hydrazinolysis conditions in semi-aqueous buffer solution. Semi-empirical calculations as well as spectroscopic results revealed the signalling mechanism of the current probe under hydrazinolysis conditions, in which hydrazine exclusively deprotected the phthalimide group by an intermediate of phthalhydrazide. Extensive screening of pH effects on the probe with the aid of proton nuclear magnetic resonance and mass spectrometry supported the distinctive and diverse ratiometric responses under hydrazinolysis and basic hydrolysis conditions. Time resolved photoluminescence measurements of the probe further confirmed its discernible ratiometric responses probed at respective wavelengths. A distinctive ratiometric response under basic hydrolysis conditions and a successful utilization of probe towards hydrazine detection in living cells are demonstrated. (C) 2013 Elsevier Ltd. All rights reserved.
A fully-aqueous red-fluorescent probe for selective optical sensing of Hg2+ and its application in living cells
(2016-07)
Pola, Murali Krishna
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Raju, Mandapati V. Ramakrishnam
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Lin, Chein-Ming
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Putikam, Raghunath
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Lin, Ming-Chang
A new red-fluorescent mercury ion sensor material is designed and synthesized, which is composed of at weezer-shaped hydrophilic probe containing bifurcated soft-base atoms N and S coupled with 2-dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran (TCF) unit. By virtue of the strong electron-accepting nature of TCF unit (as a push pull chromophore), this designed sensor material can selectively detect Hg2+ over various tested metal ions in a 100% aqueous medium via naked-eye and photoluminescence (PL) observations. Theoretical and time-resolved photoluminescence measurements further confirmed the selectivity and reversibility of the probe towards Hg2+ via intramolocular charge transfer mechanism in this sensor material. Moreover, the living cell tests by confocal fluorescence images of this sensor material towards Hg2+ were also investigated. Finally, distinguished absorption changes and fluorescence quenching spectral appearances allowed us to present the selective optical indicator of Hg2+ via TCF moiety for the first time. (C) 2016 Elsevier Ltd. All rights reserved.
A new pyrene-based aggregation induced ratiometric emission probe for selective detections of trivalent metal ions and its living cell application
(2015-02-01)
Singh, Ashutosh
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Singh, Ravinder
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Shellaiah, Muthaiah
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Prakash, Epperla Chandra
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Chang, Huan-Cheng
We synthesize a newly designed pyrene-based fluorescent probe FBP containing a Schiff base and amide linkage as a receptor, which demonstrates selective UV-vis and fluorometric detections of trivalent metal ions in various semi-aqueous solution conditions. Interestingly, by increasing water contents the aggregation induced ratiometric emissions (AIREs) of photoluminescence in FBP solutions (in DMSO/H2O) are enhanced from blue to green due to the excimer formation of the aggregates in water. In addition, the enhanced aggregated ratiometric green fluorescence behaviors of FBP in the presence of trivalent metal ions (Al3+, Fe3+, and Cr3+) are observed in semi-aqueous solutions as well as the solid state, which is well utilized to detect trivalent metal ions in living cells. Further verification of the probe detections, their interactions toward trivalent metal ions are well characterized by the time-resolved photoluminescence (TRPL) of spectroscopic responses as well as theoretical calculations. Therefore, we believe the application of such a new AIRE-based approach is first reported among Schiff base and pyrene-based fluorescentchemo- and bio-sensors. (C) 2014 Elsevier B.V. All rights reserved.
Carbon structure in nanodiamonds elucidated from Raman spectroscopy
(2017-09-01)
Korepanov, Vitaly I.
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Hamaguchi, Hiro-o
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Osawa, Eiji
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Ermolenkov, Vladimir
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Lednev, Igor K.
Despite their name, nanodiamonds (ND) are comprised of a complex interplay of different carbon phases. The diamond core is surrounded by the shell consisting mostly of disordered sp(3) carbon and graphene-like carbon (GLC). The complex structure makes the characterization of nanodiamonds (ND) a difficult challenge. Recent development of many varieties of NDs for different applications demands quick and reliable characterization of the content of various carbon fragments, as well as the estimation of the diamond core size. In this work, we apply Raman spectroscopy to study the structure of nanodiamonds from different origins, including those produced by detonation (DND), high pressure high temperature synthesis (HPHT), and pulsed laser irradiation (LND). The relative content of GLC, disordered and surface carbon can be easily determined from Raman spectra. In particular, we show how the content of different structure fragments is changed upon de-agglutination, surface oxidation and ion irradiation. We also compare the different ND production methods in terms of the structural uniformity of the nanoparticles. Raman spectroscopy provides unique quantitative tool for ND characterization; we believe that the present data will be useful for understanding the structure of diamond nanoparticles, and will provide the background for obtaining the NDs with desired properties. (C) 2017 Elsevier Ltd. All rights reserved.
Dissociation of heme from gaseous myoglobin ions studied by infrared multiphoton dissociation spectroscopy and Fourier-transform ion cyclotron resonance mass spectrometry
(2006-10-07)
Wang, Yi-Sheng
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Sabu, Sahadevan
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Wei, Shih-Chia
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Kao, C. -M. Josh
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Kong, Xianglei
Detachment of heme prosthetic groups from gaseous myoglobin ions has been studied by collision-induced dissociation and infrared multiphoton dissociation in combination with Fourier-transform ion cyclotron resonance mass spectrometry. Multiply charged holomyoglobin ions (hMb(n+)) were generated by electrospray ionization and transferred to an ion cyclotron resonance cell, where the ions of interest were isolated and fragmented by either collision with Ar atoms or irradiation with 3 mu m photons, producing apomyoglobin ions (aMb(n+)). Both charged heme loss (with [Fe(III)-heme](+) and aMb((n-1)+) as the products) and neutral heme loss (with [Fe(II)-heme] and aMb(n+) as the products) were detected concurrently for hMb(n+) produced from a myoglobin solution pretreated with reducing reagents. By reference to E-a=0.9 eV determined by blackbody infrared radiative dissociation for charged heme loss of ferric hMb(n+), an activation energy of 1.1 eV was deduced for neutral heme loss of ferrous hMb(n+) with n=9 and 10. (c) 2006 American Institute of Physics.
Excitation properties of the H3 defect center in diamond: A theoretical study
(2009-06-16)
Lin, Chih-Kai
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Chang, Huan-Cheng
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Hayashi, M.
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Lin, S. H.
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應用化學系
Theoretical calculations on electronic excitation energies and absorption cross sections of the H3 defect center in diamond were performed. We constructed model clusters with up to 180 atoms to imitate the local environment of the defect center and conducted first-principle calculations. TD-DFT predicted most accurately vertical excitation energy, whereas TD-HF and CIS provided rough estimations for one- and two-photon absorption cross sections. It was found that a model cluster with a diameter larger than similar to 1.0 nm is required, while relatively low-cost level of theory and basis set are sufficient for characterizing the excitation properties. (c) 2009 Elsevier B.V. All rights reserved.
Labeling of neuronal differentiation and neuron cells with biocompatible fluorescent nanodiamonds
(2014-05-16)
Hsu, Tzu-Chia
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Liu, Kuang-Kai
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Chang, Huan-Cheng
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Hwang, Eric
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Chao, Jui-I
Nanodiamond is a promising carbon nanomaterial developed for biomedical applications. Here, we show fluorescent nanodiamond (FND) with the biocompatible properties that can be used for the labeling and tracking of neuronal differentiation and neuron cells derived from embryonal carcinoma stem (ECS) cells. The fluorescence intensities of FNDs were increased by treatment with FNDs in both the mouse P19 and human NT2/D1 ECS cells. FNDs were taken into ECS cells; however, FNDs did not alter the cellular morphology and growth ability. Moreover, FNDs did not change the protein expression of stem cell marker SSEA-1 of ECS cells. The neuronal differentiation of ECS cells could be induced by retinoic acid (RA). Interestingly, FNDs did not affect on the morphological alteration, cytotoxicity and apoptosis during the neuronal differentiation. Besides, FNDs did not alter the cell viability and the expression of neuron-specific marker beta-III-tubulin in these differentiated neuron cells. The existence of FNDs in the neuron cells can be identified by confocal microscopy and flow cytometry. Together, FND is a biocompatible and readily detectable nanomaterial for the labeling and tracking of neuronal differentiation process and neuron cells from stem cells.
One- and two-photon absorption properties of diamond nitrogen-vacancy defect centers: A theoretical study
(2008-09-28)
Lin, Chih-Kai
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Wang, Yi-Hsieh
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Chang, Huan-Cheng
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Hayashi, M.
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Lin, S. H.
The negatively charged nitrogen-vacancy defect center, (NV)(-), in diamond has been investigated theoretically for its one-and two-photon absorption properties involving the first excited state with the (3)A(2) -> (3)E transition. Time-dependent density functional theory (TD-DFT), configuration interaction with single excitation (CIS), and complete active space self-consistent field (CASSCF) were employed in this investigation along with the 6-31G(d) basis set. Diamond lattice models containing 24-104 carbon atoms were constructed to imitate the local environment of the defect center. TD-DFT calculations in large molecular cluster models (with 85 or more carbon atoms) predicted the vertical excitation energy quite consistent with the experimental absorption maximum. CASSCF calculations were feasible only for small cluster models (less than 50 carbon atoms) but yielded one-photon absorption (OPA) and two-photon absorption (TPA) cross sections somewhat larger than the experimental values obtained with linearly polarized incident light [T.-L. Wee et al., J. Phys. Chem. A 111, 9379 (2007)]. CIS calculations in larger cluster models showed a systematic overestimation of the excitation energy while just slightly underestimated the OPA cross section and overestimated the TPA cross section. The agreements between calculations and measurements suggest that the computational approaches established in this work are applicable to explore the optical properties of related defect centers in diamond as well. (C) 2008 American Institute of Physics.
The effect of fluorescent nanodiamonds on neuronal survival and morphogenesis
(2014-11-05)
Huang, Yung-An
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Kao, Chun-Wei
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Liu, Kuang-Kai
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Huang, Hou-Syun
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Chiang, Ming-Han
Nanodiamond (ND) has emerged as a promising carbon nanomaterial for therapeutic applications. In previous studies, ND has been reported to have outstanding biocompatibility and high uptake rate in various cell types. ND containing nitrogen-vacancy centers exhibit fluorescence property is called fluorescent nanodiamond (FND), and has been applied for bio-labeling agent. However, the influence and application of FND on the nervous system remain elusive. In order to study the compatibility of FND on the nervous system, neurons treated with FNDs in vitro and in vivo were examined. FND did not induce cytotoxicity in primary neurons from either central (CNS) or peripheral nervous system (PNS); neither did intracranial injection of FND affect animal behavior. The neuronal uptake of FNDs was confirmed using flow cytometry and confocal microscopy. However, FND caused a concentration-dependent decrease in neurite length in both CNS and PNS neurons. Time-lapse live cell imaging showed that the reduction of neurite length was due to the spatial hindrance of FND on advancing axonal growth cone. These findings demonstrate that FNDs exhibit low neuronal toxicity but interfere with neuronal morphogenesis, and should be taken into consideration when applications involve actively growing neurites (e.g. nerve regeneration).
The long-term stability and biocompatibility of fluorescent nanodiamond as an in vivo contrast agent
(2012-11-01)
Vaijayanthimala, V.
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Cheng, Po-Yun
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Yeh, Shih-Hua
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Liu, Kuang-Kai
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Hsiao, Cheng-Hsiang
Nanocarbon is a promising type of biomaterial for diagnostic and therapeutic applications. Fluorescent nanodiamond (FND) containing nitrogen-vacancy centers as built-in fluorophores is a new addition to the nanocarbon family. Here, we study the long-term stability and biocompatibility of 100-nm FNDs in rats through intraperitoneal injection over 5 months and develop the potential application of this biomaterial for sentinel lymph node mapping in a mouse model. From both in vivo and ex vivo fluorescence imaging as well as transmission electron microscopy, we found that the intradermally administered FND particles can be drained from the injection sites by macrophages and selectively accumulated in the axillary lymph nodes of the treated mice. Our measurements of water consumption, fodder consumption, body weight, and organ index showed no significant difference between control and FND-treated groups of the rats. Histopathological analysis of various tissues and organs indicated that FNDs are non-toxic even when a large quantity, up to 75 mg/kg body weight, of the particles was administered intraperitoneally to the living animals. With the properties of wide-ranging biocompatibility and perfect chemical and photophysical stability, FND is well suited for use as a contrast agent for long-term in vivo imaging. (c) 2012 Elsevier Ltd. All rights reserved.
Theoretical treatments of ultrafast electron transfer from adsorbed dye molecule to semiconductor nanocrystalline surface
(2006-10-21)
Liang, Kuo Kan
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Lin, Chih-Kai
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Chang, Huan-Cheng
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Hayashi, Michitoshi
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Lin, Sheng Hsien
In studying ultrafast electron transfer from a dye molecule to a nanosized semiconductor particle, pump-probe experiments are commonly used. In this system the electron transfer (ET) rate is faster than vibrational relaxation so that the ET rate should be described by a single-level rate constant and the probing signal (often in the form of time-resolved spectra) contains the contribution from the dynamics of both population and coherence (i.e., wave packet). In this paper, we shall present the theoretical treatments for femtosecond time-resolved pump-probe experiment and the dynamics of population and coherence by the density matrix method, and the calculation of single-level ET rate constant involved in a pump-probe experiment. As an application, we show the theoretical results using parameters extracted from experiments on a specific dye/semiconductor system. (c) 2006 American Institute of Physics.
Ubiquitin-coated nanodiamonds bind to autophagy receptors for entry into the selective autophagy pathway
(2017-01-01)
Liu, Kuang-Kai
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Qiu, Wei-Ru
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Raj, Emmanuel Naveen
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Liu, Huei-Fang
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Huang, Hou-Syun
Selective macroautophagy/autophagy plays a pivotal role in the processing of foreign pathogens and cellular components to maintain homeostasis in human cells. To date, numerous studies have demonstrated the uptake of nanoparticles by cells, but their intracellular processing through selective autophagy remains unclear. Here we show that carbon-based nanodiamonds (NDs) coated with ubiquitin (Ub) bind to autophagy receptors (SQSTM1 [sequestosome 1], OPTN [optineurin], and CALCOCO2/NDP52 [calcium binding and coiled-coil domain 2]) and are then linked to MAP1LC3/LC3 (microtubule-associated protein 1 light chain 3) for entry into the selective autophagy pathway. NDs are ultimately delivered to lysosomes. Ectopically expressed SQSTM1-green fluorescence protein (GFP) could bind to the Ub-coated NDs. By contrast, the Ub-associated domain mutant of SQSTM1 (UBA)-GFP did not bind to the Ub-coated NDs. Chloroquine, an autophagy inhibitor, prevented the ND-containing autophagosomes from fusing with lysosomes. Furthermore, autophagy receptors OPTN and CALCOCO2/NDP52, involved in the processing of bacteria, were found to be involved in the selective autophagy of NDs. However, ND particles located in the lysosomes of cells did not induce mitotic blockage, senescence, or cell death. Single ND clusters in the lysosomes of cells were observed in the xenografted human lung tumors of nude mice. This study demonstrated for the first time that Ub-coated nanoparticles bind to autophagy receptors for entry into the selective autophagy pathway, facilitating their delivery to lysosomes.
YY Ubiquitin-coated nanodiamonds bind to autophagy receptors for entry into the selective autophagy pathway
(2017)
Liu, Kuang-Kai
;
Qiu, Wei-Ru
;
Raj, Emmanuel Naveen
;
Liu, Huei-Fang
;
Huang, Hou-Syun
Selective macroautophagy/autophagy plays a pivotal role in the processing of foreign pathogens and cellular components to maintain homeostasis in human cells. To date, numerous studies have demonstrated the uptake of nanoparticles by cells, but their intracellular processing through selective autophagy remains unclear. Here we show that carbon-based nanodiamonds (NDs) coated with ubiquitin (Ub) bind to autophagy receptors (SQSTM1 [sequestosome 1], OPTN [optineurin], and CALCOCO2/NDP52 [calcium binding and coiled-coil domain 2]) and are then linked to MAP1LC3/LC3 (microtubule-associated protein 1 light chain 3) for entry into the selective autophagy pathway. NDs are ultimately delivered to lysosomes. Ectopically expressed SQSTM1-green fluorescence protein (GFP) could bind to the Ub-coated NDs. By contrast, the Ub-associated domain mutant of SQSTM1 (UBA)-GFP did not bind to the Ub-coated NDs. Chloroquine, an autophagy inhibitor, prevented the ND-containing autophagosomes from fusing with lysosomes. Furthermore, autophagy receptors OPTN and CALCOCO2/NDP52, involved in the processing of bacteria, were found to be involved in the selective autophagy of NDs. However, ND particles located in the lysosomes of cells did not induce mitotic blockage, senescence, or cell death. Single ND clusters in the lysosomes of cells were observed in the xenografted human lung tumors of nude mice. This study demonstrated for the first time that Ub-coated nanoparticles bind to autophagy receptors for entry into the selective autophagy pathway, facilitating their delivery to lysosomes.
YY Ubiquitin-coated nanodiamonds bind to autophagy receptors for entry into the selective autophagy pathway
(2017)
Liu, Kuang-Kai
;
Qiu, Wei-Ru
;
Raj, Emmanuel Naveen
;
Liu, Huei-Fang
;
Huang, Hou-Syun
Selective macroautophagy/autophagy plays a pivotal role in the processing of foreign pathogens and cellular components to maintain homeostasis in human cells. To date, numerous studies have demonstrated the uptake of nanoparticles by cells, but their intracellular processing through selective autophagy remains unclear. Here we show that carbon-based nanodiamonds (NDs) coated with ubiquitin (Ub) bind to autophagy receptors (SQSTM1 [sequestosome 1], OPTN [optineurin], and CALCOCO2/NDP52 [calcium binding and coiled-coil domain 2]) and are then linked to MAP1LC3/LC3 (microtubule-associated protein 1 light chain 3) for entry into the selective autophagy pathway. NDs are ultimately delivered to lysosomes. Ectopically expressed SQSTM1-green fluorescence protein (GFP) could bind to the Ub-coated NDs. By contrast, the Ub-associated domain mutant of SQSTM1 (UBA)-GFP did not bind to the Ub-coated NDs. Chloroquine, an autophagy inhibitor, prevented the ND-containing autophagosomes from fusing with lysosomes. Furthermore, autophagy receptors OPTN and CALCOCO2/NDP52, involved in the processing of bacteria, were found to be involved in the selective autophagy of NDs. However, ND particles located in the lysosomes of cells did not induce mitotic blockage, senescence, or cell death. Single ND clusters in the lysosomes of cells were observed in the xenografted human lung tumors of nude mice. This study demonstrated for the first time that Ub-coated nanoparticles bind to autophagy receptors for entry into the selective autophagy pathway, facilitating their delivery to lysosomes.