Browsing by Author "Chang, Shwu-Jen"

Now showing 1 - 4 of 4

Cartilage Tissue-Mimetic Pellets with Multifunctional Magnetic Hyaluronic Acid-Graft-Amphiphilic Gelatin Microcapsules for Chondrogenic Stimulation
(2020-04-01)
Hou, Kai-Ting
;
Liu, Ting-Yu
;
Chiang, Min-Yu
;
Chen, Chun-Yu
;
Chang, Shwu-Jen
Articular cartilage defect is a common disorder caused by sustained mechanical stress. Owing to its nature of avascular, cartilage had less reconstruction ability so there is always a need for other repair strategies. In this study, we proposed tissue-mimetic pellets composed of chondrocytes and hyaluronic acid-graft-amphiphilic gelatin microcapsules (HA-AGMCs) to serve as biomimetic chondrocyte extracellular matrix (ECM) environments. The multifunctional HA-AGMC with specific targeting on CD44 receptors provides excellent structural stability and demonstrates high cell viability even in the center of pellets after 14 days culture. Furthermore, with superparamagnetic iron oxide nanoparticles (SPIOs) in the microcapsule shell of HA-AGMCs, it not only showed sound cell guiding ability but also induced two physical stimulations of static magnetic field(S) and magnet-derived shear stress (MF) on chondrogenic regeneration. Cartilage tissue-specific gene expressions of Col II and SOX9 were upregulated in the present of HA-AGMC in the early stage, and HA-AGMC+MF+S held the highest chondrogenic commitments throughout the study. Additionally, cartilage tissue-mimetic pellets with magnetic stimulation can stimulate chondrogenesis and sGAG synthesis.
Direct Reprogramming of Human Suspension Cells into Mesodermal Cell Lineages via Combined Magnetic Targeting and Photothermal Stimulation by Magnetic Graphene Oxide Complexes
(2017-08-25)
Chiang, Min-Yu
;
Lin, Yi-Zhen
;
Chang, Shwu-Jen
;
Shyu, Woei-Cherng
;
Lu, Huai-En
Suspension cells can provide a source of cells for cellular reprogramming, but they are difficult to transfect by nonviral vectors. An efficient and safe nonviral vector (GO-Fe3O4-PEI complexes) based on iron oxide nanoparticle (Fe3O4)-decorated graphene oxide (GO) complexed with polyethylenimine (PEI) for the first time is developed for delivering three individual episomal plasmids (pCXLE-hOCT3/4-shp53, pCXLE-hSK, and pCXLE-hUL) encoding pluripotent-related factors of Oct3/4, shRNA against p53, Sox2, Klf4, L-Myc, and Lin28 into human peripheral blood mononuclear cells (PBMCs) simultaneously. The combined treatment of magnetic stirring and near-infrared (NIR)-laser irradiation, which can promote contact between the complexes and floating cells and increase the cell membrane permeability, respectively, is used to conduct multiple physical stimulations for suspension PBMCs transfection. The PCR analysis shows that the combinatorial effect of magnetic targeting and photothermal stimulation obviously promoted the transfection efficiency of suspension cells. The transfected cells show positive expression of the pluripotency markers, including Nanog, Oct4, and Sox2, and have potential to differentiate into mesoderm and ectoderm cells. The results demonstrate that the GO-Fe3O4-PEI complex provides a safe, convenient, and efficient tool for reprogramming PBMCs into partially induced pluripotent stem cells, which are able to rapidly transdifferentiate into mesodermal lineages without full reprogramming.
Magnetic amphiphilic gelatin microcapsules for cartilage tissue engineering
(2017-01-01)
Hou, Kai-Ting
;
Chang, Shwu-Jen
;
Chen, San-Yuan
;
材料科學與工程學系
;
Department of Materials Science and Engineering
To evaluate a multifunctional microcapsule for cartilage tissue engineering, we developed a brand new amphiphilic material grafting Hyaluronic acid (HA) on hexanoic anhydride modified gelatin. The material formed microcapsules (HA-AGMC) loaded with superparamagnetic iron oxide (SPIO) and TGF-beta(1) can stimulate chondrogenic differentiation by the combination of biochemical and biophysical stimulation.
Using the interplay of magnetic guidance and controlled TGF-beta release from protein-based nanocapsules to stimulate chondrogenesis
(2018-01-01)
Chiang, Chih-Sheng
;
Chen, Jian-Yi
;
Chiang, Min-Yu
;
Hou, Kai-Ting
;
Li, Wei-Ming
Introduction: Stimulating the proliferation and differentiation of chondrocytes for the regeneration of articular cartilage is a promising strategy, but it is currently ineffective. Although both physical stimulation and growth factors play important roles in cartilage repair, their interplay remains unclear and requires further investigation. In this study, we aimed to clarify their contribution using a magnetic drug carrier that not only can deliver growth factors but also provide an external stimulation to cells in the two-dimensional environment. Materials and methods: We developed a nanocapsule ( transforming growth factor-beta 1 [TGF-beta 1]lo aded magnetic amphiphilic gelatin nanocapsules [MAGNCs]; TGF-beta 1@MAGNCs) composed of hexanoic-anhydride-grafted gelatin and iron oxide nanoparticles to provide a combination treatment of TGF-beta 1 and magnetically induced physical stimuli. With the expression of Arg-Gly-Asp peptide in the gelatin, the TGF-beta 1@MAGNCs have an inherent affinity for chondrogenic ATDC5 cells. Results: In the absence of TGF-beta 1, ATDC5 cells treated with a magnetic field show significantly upregulated Col2a1 expression. Moreover, TGF-beta 1 slowly released from biodegradable TGF-beta 1@MAGNCs further improves the differentiation with increased expression of Col2a1 and Aggrecan. Conclusion: Our study shows the time-dependent interplay of physical stimuli and growth factors on chondrogenic regeneration, and demonstrates the promising use of TGF-beta 1@MAGNCs for articular cartilage repair.