Browsing by Author "Chang, Yung-Fu"

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Muscle developmental defects in heterogeneous nuclear Ribonucleoprotein A1 knockout mice
(2017-01-01)
Liu, Ting-Yuan
;
Chen, Yu-Chia
;
Jong, Yuh-Jyh
;
Tsai, Huai-Jen
;
Lee, Chien-Chin
Heterogeneous ribonucleoprotein A1 (hnRNP A1) is crucial for regulating alternative splicing. Its integrated function within an organism has not, however, been identified. We generated hnRNP A1 knockout mice to study the role of hnRNPA1 in vivo. The knockout mice, hnRNP A1(-/-), showed embryonic lethality because of muscle developmental defects. The blood pressure and heart rate of the heterozygous mice were higher than those of the wild-type mice, indicating heart function defects. We performed mouse exon arrays to study the muscle development mechanism. The processes regulated by hnRNP A1 included cell adhesion and muscle contraction. The expression levels of muscle development-related genes in hnRNPA1(+/-) mice were significantly different from those in wild-type mice, as detected using qRT-PCR. We further confirmed the alternative splicing patterns of muscle development-related genes including mef2c, lrrfip1, usp28 and abcc9. Alternative mRNA isoforms of these genes were increased in hnRNP A1(+/-) mice compared with wild-type mice. Furthermore, we revealed that the functionally similar hnRNP A2/B1 did not compensate for the expression of hnRNP A1 in organisms. In summary, our study demonstrated that hnRNP A1 plays a critical and irreplaceable role in embryonic muscle development by regulating the expression and alternative splicing of muscle-related genes.
Muscle developmental defects in heterogeneous nuclear Ribonucleoprotein A1 knockout mice
(2017-01)
Liu, Ting-Yuan
;
Chen, Yu-Chia
;
Jong, Yuh-Jyh
;
Tsai, Huai-Jen
;
Lee, Chien-Chin
Heterogeneous ribonucleoprotein A1 (hnRNP A1) is crucial for regulating alternative splicing. Its integrated function within an organism has not, however, been identified. We generated hnRNP A1 knockout mice to study the role of hnRNPA1 in vivo. The knockout mice, hnRNP A1(-/-), showed embryonic lethality because of muscle developmental defects. The blood pressure and heart rate of the heterozygous mice were higher than those of the wild-type mice, indicating heart function defects. We performed mouse exon arrays to study the muscle development mechanism. The processes regulated by hnRNP A1 included cell adhesion and muscle contraction. The expression levels of muscle development-related genes in hnRNPA1(+/-) mice were significantly different from those in wild-type mice, as detected using qRT-PCR. We further confirmed the alternative splicing patterns of muscle development-related genes including mef2c, lrrfip1, usp28 and abcc9. Alternative mRNA isoforms of these genes were increased in hnRNP A1(+/-) mice compared with wild-type mice. Furthermore, we revealed that the functionally similar hnRNP A2/B1 did not compensate for the expression of hnRNP A1 in organisms. In summary, our study demonstrated that hnRNP A1 plays a critical and irreplaceable role in embryonic muscle development by regulating the expression and alternative splicing of muscle-related genes.
Performance Enhancement of Ultraviolet Light-Emitting Diodes by Incorporating a Thin Al(In)GaN Interlayer in Multiquantum-Well Region
(2015-07-01)
Liu, Jia-Zhe
;
Lin, Chung-Hsiang
;
Lee, Kang-Yuan
;
Wang, Yu-Li
;
Liao, Chien-Lan
In this paper, the performance improvement of 380-nm InGaN/AlGaN ultraviolet light-emitting diodes (UV-LEDs) is investigated by incorporating an undoped Al(In)GaN thin interlayer between the InGaN well and AlGaN barrier in multiquantum-well (MQW) active region. By inserting the graded-composition AlGaN and AlInGaN thin interlayers, the light output powers of UV-LEDs are significantly increased by 70% and 105% at 20 mA, respectively, as compared with the LED without the interlayer. Remarkable efficiency enhancement in the UV-LEDs with graded-composition AlGaN and AlInGaN interlayers is mainly attributed to the further improvement of the electron confinement and hole injection with more uniform distribution in the MQW active region. Besides, photoluminescence and atomic force microscope analyses indicate that the MQW quality can be enhanced by incorporating a graded-composition AlInGaN thin interlayer in the MQW active region of UV-LEDs.