Browsing by Author "Chen, CG"

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CaNdt80 is involved in drug resistance in Candida albicans by regulating CDR1
(2004-12-01)
Chen, CG
;
Yang, YL
;
Shih, HI
;
Su, CL
;
Lo, HJ
Overexpression of CDR1, an efflux pump, is one of the major mechanisms contributing to drug resistance in Candida albicans. CDR1 p-lacZ was constructed and transformed into a Saccharomyces cerevisiae strain so that the lacZ gene could be used as the reporter to monitor the activity of the CDR1 promoter. Overexpression of CaNDT80, the C albicans homolog of S. cerevisiae NDT80, increases the beta-galactosidase activity of the CDR1 p-lacZ construct in S. cerevisiae. Furthermore, mutations in CaNDT80 abolish the induction of CDR1 expression by antifungal agents in C albicans. Consistently, the Candt80/Candt80 mutant is also more susceptible to antifungal drugs than the wild-type strain. Thus, the gene for CaNdt80 may be the first gene among the regulatory factors involved in drug resistance in C. albicans whose function has been identified.
Efg1 involved in drug resistance by regulating the expression of ERG3 in Candida albicans
(2005-03-01)
Lo, HJ
;
Wang, JS
;
Lin, CY
;
Chen, CG
;
Hsiao, TY
The ERG3 gene in Candida albicans was identified as a gene whose mRNA level was higher in the cph1/cph1 efg1/efg1 double mutant than in the wild-type cells. Further study showed that Efg1, but not Cph1, negatively regulated ERG3. Mutations in EFG1 consistently increased the susceptibility of the cells to antifungal agents.
Identification of trans-regulatory factors of the efflux pumps involved in drug resistance in Candida albicans.
(2003-07-01)
Chen, CG
;
Shih, HI
;
Yang, YL
;
Lo, HJ
;
生物科技學系