Browsing by Author "Chen, Cheng-cheung"

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Chondroitin sulfate-stabilized silver nanoparticles: Improved synthesis and their catalytic, antimicrobial, and biocompatible activities
(2018-03-02)
Young, Jenn-jong
;
Cheng, Kuang-ming
;
Young, Yen-an
;
Chen, Xin-an
;
Chen, Yu-hao
Herein, we describe an improved procedure for the green synthesis of chondroitin sulfate stabilized silver nanoparticles (ChS-AgNPs). Glucose was used as a reducing agent under alkaline conditions to obtain a small particle size (<10 nm), and the reduction was complete within one hour at room temperature. The concentration of NaOH affected the reaction rate, formation yield, and particle size of ChS-AgNPs. The formation of AgNPs was confirmed using UV-vis, TEM, XRD, and XPS. ChS-AgNPs showed excellent catalytic activities in the reduction of 4-nitrophenol by NaBH4, and the reaction rate increased linearly with increasing catalyst amounts. The antimicrobial activities of ChS-AgNPs against A. baumannii (including multidrug-resistant strains), E. coli, P. aeruginosa, and S. aureus were evaluated using the broth microdilution method. Finally, from the morphological observations and cell cycle analysis of L929 cells, we found that ChS-AgNPs exhibited antimicrobial and biocompatible activities. (c) 2017 Elsevier Ltd. All rights reserved.
Novel protein-loaded chondroitin sulfate-N-[(2hydroxy-3-trimethylammonium)propyl]chitosan nanoparticles with reverse zeta potential: preparation, characterization, and ex vivo assessment
(2015-01-01)
Tsai, Tsung-Neng
;
Yen, Hui-Ju
;
Chen, Cheng-cheung
;
Chen, Ying-chuan
;
Young, Yen-an
A facile polyelectrolyte complexation method for the preparation of both positively and negatively surface charged nanoparticles composed of chondroitin sulfate (ChS) and N-[(2-hydroxy-3-trimethylammonium)-propyl]chitosan (HTCC) is reported. Production of ChS-HTCC nanoparticles with reverse zeta potential was easily controlled by varying the ChS/HTCC mass ratio. The encapsulation efficiency increased with the increase in initial FITC-BSA concentration in positively charged NPs and reached 75%. However, a maximum of 20% encapsulation efficiency was achieved in the case of negatively charged NPs. In vitro release studies of positively charged ChS-HTCC NPs showed a small burst effect followed by a continued and controlled release. Both charges of ChS-HTCC NPs showed no cytotoxicity in HUVECs. The confocal images showed that ChS-HTCC NPs of both charges can be incorporated and retained by the A549 cells. Flow cytometric analysis data demonstrated that ChS-HTCC NPs of both charges were detected in more than 80% of the A549 cells.