Browsing by Author "Chen, Chien-Jen"

Now showing 1 - 3 of 3

Idi1 and Hmgcs2 Are Affected by Stretch in HL-1 Atrial Myocytes
(2018-12-01)
Fang, Chih-Yuan
;
Chen, Mien-Cheng
;
Chang, Tzu-Hao
;
Wu, Chia-Chen
;
Chang, Jen-Ping
Background: Lipid expression is increased in the atrial myocytes of mitral regurgitation (MR) patients. This study aimed to investigate key regulatory genes and mechanisms of atrial lipotoxic myopathy in MR. Methods: The HL-1 atrial myocytes were subjected to uniaxial cyclic stretching for eight hours. Fatty acid metabolism, lipoprotein signaling, and cholesterol metabolism were analyzed by PCR assay (168 genes). Results: The stretched myocytes had significantly larger cell size and higher lipid expression than non-stretched myocytes (all p < 0.001). Fatty acid metabolism, lipoprotein signaling, and cholesterol metabolism in the myocytes were analyzed by PCR assay (168 genes). In comparison with their counterparts in non-stretched myocytes, seven genes in stretched monocytes (Idi1, Olr1, Nr1h4, Fabp2, Prkag3, Slc27a5, Fabp6) revealed differential upregulation with an altered fold change >1.5. Nine genes in stretched monocytes (Apoa4, Hmgcs2, Apol8, Srebf1, Acsm4, Fabp1, Acox2, Acsl6, Gk) revealed differential downregulation with an altered fold change <0.67. Canonical pathway analysis, using Ingenuity Pathway Analysis software, revealed that the only genes in the superpathway of cholesterol biosynthesis were Idi1 (upregulated) and Hmgcs2 (downregulated). The fraction of stretched myocytes expressing Nile red was significantly decreased by RNA interference of Idi1 (p < 0.05) and was significantly decreased by plasmid transfection of Hmgcs2 (p = 0.004). Conclusions: The Idi1 and Hmgcs2 genes have regulatory roles in atrial lipotoxic myopathy associated with atrial enlargement.
Influence of electrode configuration on the heat transfer performance of a LED heat source
(2014-10-01)
Chen, Ing Youn
;
Chen, Chien-Jen
;
Wang, Chi-Chuan
;
機械工程學系
;
Department of Mechanical Engineering
In this study, the effects of electrode configuration on the cooling of LED are presented. A total of six needle type electrodes, including sharp, 0.08, 0.12, 0.37, 0.49 R and flat tip, are used to generate ionic wind for cooling of the LED. The effects of electrode configuration, vertical separation height, and tilt angle on the cooling performance of a LED are reported in this study. It is found that the thermal resistance is reduced with the supplied voltage and a maximum 50% reduction is achieved before the spark-over voltage. For the same supplied voltage, the thermal resistance with a larger vertical separation is also higher. However, the operational range is also longer. The effect of tilt angle on the cooling performance of LED depends on the supplied voltage. With a supplied voltage being less than 6.5 kV, it is found that the thermal resistance is increased when the tilt angle is reduced with a tip radius of 0.12 R. However, the trend is reversed when the supplied voltage is higher than 6.5 kV where the thermal resistance of a smaller tilt angle is lower than that of a larger tilt angle. For the same mesh ground, the effect of electrode configurations cast little influence on the final thermal resistance. All the electrodes show an approximately 50% reduction of thermal resistance in association with its original thermal resistance. However, the corresponding threshold voltage and operational voltage differs significantly. The threshold voltage is lowest for the sharp needle and is increased with the rise of tip radius. On the other hand, the corresponding operational range becomes narrower when the tip radius is increased. Yet the spark-over voltage is also insensitive to change of electrode configuration. (C) 2014 Elsevier Ltd. All rights reserved.
Influence of electrode configuration on the heat transfer performance of a LED heat source
(2014-10-01)
Chen, Ing Youn
;
Chen, Chien-Jen
;
Wang, Chi-Chuan
;
機械工程學系
;
Department of Mechanical Engineering
In this study, the effects of electrode configuration on the cooling of LED are presented. A total of six needle type electrodes, including sharp, 0.08, 0.12, 0.37, 0.49 R and flat tip, are used to generate ionic wind for cooling of the LED. The effects of electrode configuration, vertical separation height, and tilt angle on the cooling performance of a LED are reported in this study. It is found that the thermal resistance is reduced with the supplied voltage and a maximum 50% reduction is achieved before the spark-over voltage. For the same supplied voltage, the thermal resistance with a larger vertical separation is also higher. However, the operational range is also longer. The effect of tilt angle on the cooling performance of LED depends on the supplied voltage. With a supplied voltage being less than 6.5 kV, it is found that the thermal resistance is increased when the tilt angle is reduced with a tip radius of 0.12 R. However, the trend is reversed when the supplied voltage is higher than 6.5 kV where the thermal resistance of a smaller tilt angle is lower than that of a larger tilt angle. For the same mesh ground, the effect of electrode configurations cast little influence on the final thermal resistance. All the electrodes show an approximately 50% reduction of thermal resistance in association with its original thermal resistance. However, the corresponding threshold voltage and operational voltage differs significantly. The threshold voltage is lowest for the sharp needle and is increased with the rise of tip radius. On the other hand, the corresponding operational range becomes narrower when the tip radius is increased. Yet the spark-over voltage is also insensitive to change of electrode configuration. (C) 2014 Elsevier Ltd. All rights reserved.