Browsing by Author "Chen, Ching-Hung"

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Matrix metalloproteinase 2 level in human follicular fluid is a reliable marker of human oocyte maturation in in vitro fertilization and intracytoplasmic sperm injection cycles
(2015-09-04)
Yang, Wen-Jui
;
Liu, Fon-Chang
;
Hsieh, Jih-Sheng
;
Chen, Ching-Hung
;
Hsiao, Shun-Yu
Background: To determine whether matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMP-1 and TIMP-2) in human follicular fluid, have any relationships with oocyte maturation in vivo and subsequent fertilization during in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) cycles. Methods: The follicular fluids were obtained from 150 female patients undergoing IVF/ICSI cycles and a total of 1504 oocytes were retrieved for analysis. MMP-2 and MMP-9 activities were measured using zymography assay. TIMP-1 and TIMP-2 concentrations were quantitatively assessed using enzyme-linked immunosorbent assay (ELISA). Results: Human follicular fluid MMP-2 level was significantly associated with the rate of maturity of oocytes (P < 0.001). Furthermore, the MMP-2 was significantly associated with the higher fertilization rate (P < 0.01). There was no significant correlation between follicular MMP-9 and the maturation rate of oocytes. The TIMP-1 and TIMP-2 also showed no correlation with the oocyte maturation rate. Conclusions: The level of gelatinase MMP-2 in human follicular fluid might be a reliable marker of mature oocytes during IVF/ICSI cycles. Furthermore, the MMP-2 expression has a strong association with higher fertilization rate. Further studies are needed to support this theory.
MONOLITHIC NI-ELECTROPLATED MICROSTRUCTURE AND ITS APPLICATION IN MICRO MIRRORS AND MICRO ASSEMBLY
(2014-01-01)
Chiu, Yi
;
Lai, Wen-Ching
;
Chang, Chun-Hao
;
Chang, Chun-Po
;
Chen, Ching-Hung
This paper presents micro mirrors and micro assembly mechanisms fabricated by monolithic nickel electroplating. Multiple nickel structural layers were electroplated and patterned with copper as the sacrificial material. A novel side latch design was proposed and demonstrated for 3D micro assembly of micro mirrors by simple push operation of micro probes. Micro mirrors was assembled in about 15 sec in laboratory tests. The resonance frequency of the scanning mode of a stand-alone micro mirror was measured to be 4.753 kHz with a quality factor of about 470.
THz Generation and Detection on Dirac Fermions in Topological Insulators
(2013-11-01)
Luo, Chih Wei
;
Chen, Hsueh-Ju
;
Tu, Chien Ming
;
Lee, Chia Ching
;
Ku, Shin An