Browsing by Author "Chen, Chuan"

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Dark fermentation production of volatile fatty acids from glucose with biochar amended biological consortium
(2020-05-01)
Lu, Jia-Hsun
;
Chen, Chuan
;
Huang, Chihpin
;
Zhuang, Huichuan
;
Leu, Shao-Yuan
Effects of adding biochars on dark fermentation production of volatile fatty acids (VFAs) from glucose were investigated. Nine biochars were synthesized and applied, together with an activated carbon, as the testing amendment to enhance preferable fermentation. Biochars were porous materials with internal pores and excess surface functional groups, which would lead to enrichment of acetate over butyrate in the VFA production. Biochar coconut and biochar longan shell showed excess functional groups and high bulk internal crystallinity, presented 109.6% and 71.8% enrichments of acetate production, respectively. The syntrophic growth of fermentative bacteria and homoacetogens on biochar surfaces via direct interspecies electron transfer mechanism was assumed to interpret the noted enhanced acetate production. The excess functional groups on biochar surface to facilitate biofilm development and the high crystallinity of biochar bulk to ease electron transfer favored the production of acetate.
Glucose fermentation with biochar amended consortium: Sequential fermentations
(2020-05-01)
Lu, Jia-Hsun
;
Chen, Chuan
;
Huang, Chihpin
;
Leu, Shao-Yuan
;
Lee, Duu-Jong
The aim of this work was to study sequential batch fermentation of glucose with a biological consortium amended with nine different biochars or with an activated carbon. The glucose fermentation was enhanced by carbon amendment, with activated carbon being more effective than biochars as cell carriers and electron conductors between functional species. The volatile fatty acid distributions were shifted in the consumption of the produced H-2 and CO2. The types of biochars were irrelevant to glucose glycolysis and the subsequent H-2 and CO2 consumption reactions. Biofilm growth affects the detailed mechanisms occurred in fermentation broth to the yielded volatile fatty acid distributions.
Glucose fermentation with biochar-amended consortium: microbial consortium shift
(2020-01-01)
Lu, Jia-Hsun
;
Chen, Chuan
;
Huang, Chihpin
;
Lee, Duu-Jong
;
環境工程研究所
The effects of adding biochar rice husk (R), white popinee (WP), bamboo (BB), or coconut (CT) on microbial community in fermentation broths from glucose were investigated. The added biochars acted as biofilm carriers on which Sporolactobacillus spathodeae, Clostridium sensu stricto 11 sp., Clostridium sensu stricto 12 sp., Clostridium sensu stricto 1 sp., and Clostridium sensu stricto 5 sp. were enriched. Fermentation reactions substantially increased the amounts of acid-producers in biofilm. The homoacetogens, Clostridium carboxidivorans and Clostridium drakei, were identified in the biofilm in the first two batches of fermentation with biochars as electron conductors between acid-producers and homoacetogens to assist homoacetogenesis. The heterotrophic bacteria overcompeted the acid-producers in the biofilm in long-term fermentation.