Browsing by Author "Chen, Fang Ming"

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A regularly spaced and self-revealing protein ladder for anti-tag Western blot analysis
(2012-12-01)
Kao, Chien-Han
;
Cheng, Chiu-Min
;
Chuang, Kuo-Hsiang
;
Chuang, Chih-Hung
;
Tzou, Shey-Cherng
We designed a protein ladder (hereafter referred to as "Mega-tag") that contains 14 of the most commonly used epitope tags fused to molecular weight markers. The Mega-tag ladder can be simultaneously visualized when anti-tag antibodies are used to detect epitope-tagged recombinant proteins in Western blots. The logarithm of molecular weights and relative mobility of the Mega-tag protein ladder are highly correlated (R-2 = 0.997 +/- 0.00232), indicating that the dye-free Mega-tag protein ladder is accurate. It can also serve as a positive control for anti-epitope tag immunoblots. The Mega-tag protein ladder should provide a convenient and precise tool for Western blot analysis. (c) 2012 Elsevier Inc. All rights reserved.