Browsing by Author "Chen, Li-Chi"

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Formation of suspended bilayer lipid membrane between electrowetting-driven encapsulated droplets
(2014-09-01)
Fan, Shih-Kang
;
Chen, Ching-Wen
;
Lin, Yi-Ying
;
Chen, Li-Chi
;
Tseng, Fan-Gang
Using limited lipid molecules held by two water-core/oil-shell encapsulated droplets, we formed an optically observable bilayer lipid membrane (BLM) across a microfabricated aperture whose dimension was precisely determined and easily scalable. For the minute volume of a 0.1 mu L oil (n-decane) shell encapsulating a 1.5 mu L water core droplet, only 0.2 to 2.8 nmol or 0.17 to 2.4 mu g lipid was required. Microscopes and electrophysiological measurements were performed on the parallel-plate device with three major steps demonstrated: (1) manipulating self-assembled lipid monolayers at the water-oil interface of the encapsulated droplets by electrowetting-on-dielectric (EWOD), (2) forming a BLM by bringing two lipid monolayers towards the aperture and thinning down the oil film between them by Young-Laplace pressure, and (3) incorporating membrane-bound nanopores, alpha-hemolysin (alpha HL), on the BLM. This study shows the influence of the lipid concentration to the interfacial tension and EWOD. Plateau-Gibbs border and black membrane area of the BLM were optically observed, while trans-membrane electrophysiological signals were electrically recorded from a pair of Ag/AgCl electrodes. (C) 2014 AIP Publishing LLC.
Imitating Popular Photos to Select Views for an Indoor Scene
(2019-10-01)
Su, Rung-De
;
Liao, Zhe-Yo
;
Chen, Li-Chi
;
Tung, Ai-Ling
;
Wang, Yu-Shuen
Selecting informative and visually appealing views for 3D indoor scenes is beneficial for the housing, decoration, and entertainment industries. A set of views that exhibit comfort, aesthetics, and functionality of a particular scene can attract customers and facilitate business transactions. However, selecting views for an indoor scene is challenging because the system has to consider not only the need to reveal as much information as possible, but also object arrangements, occlusions, and characteristics. Since there can be many principles utilized to guide the view selection, and various principles to follow under different circumstances, we achieve the goal by imitating popular photos on the Internet. Specifically, we select the view that can optimize the contour similarity of corresponding objects to the photo. Because the selected view can be inadequate if object arrangements in the 3D scene and the photo are different, our system imitates many popular photos and selects a certain number of views. After that, it clusters the selected views and determines the view/cluster centers by the weighted average to finally exhibit the scene. Experimental results demonstrate that the views selected by our method are visually appealing.