Browsing by Author "Chen, Po-Jung"

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A High-Sensitivity and Low-Power Theranostic Nanosystem for Cell SERS Imaging and Selectively Photothermal Therapy Using Anti-EGFR-Conjugated Reduced Graphene Oxide/Mesoporous Silica/AuNPs Nanosheets
(2016-03-16)
Chen, Yu-Wei
;
Liu, Ting-Yu
;
Chen, Po-Jung
;
Chang, Po-Hsueh
;
Chen, San-Yuan
A high-sensitivity and low-power theranostic nanosystem that combines with synergistic photothermal therapy and surface-enhanced Raman scattering (SERS) mapping is constructed by mesoporous silica self-assembly on the reduced graphene oxide (rGO) nanosheets with nanogap-aligned gold nanoparticles (AuNPs) encapsulated and arranged inside the nanochannels of the mesoporous silica layer. Rhodamine 6G (R6G) as a Raman reporter is then encapsulated into the nanochannels and anti-epidermal growth factor receptor (EGFR) is conjugated on the nanocomposite surface, defined as anti-EGFR-PEG-rGO@CPSS-Au-R6G, where PEG is polyethylene glycol and CPSS is carbon porous silica nanosheets. SERS spectra results show that rGO@CPSS-Au-R6G enhances 5 x 10(6) magnification of the Raman signals and thus can be applied in the noninvasive cell tracking. Furthermore, it displays high sensitivity (detection limits: 10(-8) M R6G solution) due to the "hot spots" effects by the arrangements of AuNPs in the nanochannels of mesoporous silica. The highly selective targeting of overexpressing EGFR lung cancer cells (A549) is observed in the anti-EGFR-PEG-rGO@CPSS-Au-R6G, in contrast to normal cells (MRC-5). High photothermal therapy efficiency with a low power density (0.5 W cm(-2)) of near-infrared laser can be achieved because of the synergistic effect by conjugated AuNPs and rGO nanosheets. These results demonstrate that the anti-EGFR-PEG-rGO@CPSS-Au-R6G is an excellent new theranostic nanosystem with cell targeting, cell tracking, and photothermal therapy capabilities.
A novel multifunctional nano-platform with enhanced anti-cancer and photoacoustic imaging modalities using gold-nanorod-filled silica nanobeads
(2013)
Chen, Po-Jung
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Hu, Shang-Hsiu
;
Fan, Chih-Tai
;
Li, Meng-Lin
;
Chen, You-Yin
The novel nano-seaurchin structure is characteristic of high-density and well-dispersed gold nanorods in one mesoporous silica nanobead. This nanoplatform provided increased photothermal stability, stable photoacoustic signal and highly efficient hyperthermia effect both in vitro and in vivo, indicating a powerful theranostic modality.
Core-Shell Nanocapsules Stabilized by Single-Component Polymer and Nanoparticles for Magneto-Chemotherapy/Hyperthermia with Multiple Drugs
(1970-01-01)
Hu, Shang-Hsiu
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Liao, Bang-Jie
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Chiang, Chin-Sheng
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Chen, Po-Jung
;
Chen, I-Wei
Geometrical confinement of quantum dots in porous nanobeads with ultraefficient fluorescence for cell-specific targeting and bioimaging
(2012)
Chen, Po-Jung
;
Hu, Shang-Hsiu
;
Hung, Wen-Ting
;
Chen, San-Yuan
;
Liu, Dean-Mo
In this study, a new imaging mesoporous nanoparticle is reported with exceptionally efficient and stable fluorescence emission, which was constructed by embedding and stabilizing quantum dots (QDs) of different sizes into porous nanobeads, following a lipid-PEG(2000)-COOH coating which was further encoded with cRGD targeting peptide through biotin-streptavidin bridges. The mesopores had similar to 10 nm diameter, were chemically modified, and facilitated internalization and stabilization of the QDs within the nanobeads upon the preparation protocol. Their outstanding optical contrasts render the highly fluorescent QDs as ideal fluorophores for wavelength-and-intensity multiplex color coding. The QDs tagged nanobeads showed optically strong and chemically stable imaging capability, both in vitro and in vivo, indicating powerful contrast modality among other alternatives. The cRGD-encoded lipid coated QDs tagged nanobeads (cRGD-encoded LQNBs) exhibited significantly increased alpha(v)beta(3)-expressing cell targeting toward MCF-7 breast cancer cells over the alpha(v)beta(3)-low expressing in HeLa cervix cancer cells, as confirmed by confocal laser scanning microscopy and flow cytometry. In MCF-7 xenograft nude mice, the cRGD-encoded LQNBs revealed prolonged accumulation time at the tumor site. In addition, the QNBs also demonstrated relatively high cell viability as compared to 3-mercaptopropionic acid (MPA)-functionalized QDs, indicating a successful design of highly-cytocompatible nanoparticulate platform capable of providing cell-specific targeting and nano-imaging modalities for biomedical applications.
Multifunctional magnetically removable nanogated lids of Fe(3)O(4)-capped mesoporous silica nanoparticles for intracellular controlled release and MR imaging
(2011-01-01)
Chen, Po-Jung
;
Hu, Shang-Hsiu
;
Hsiao, Chi-Sheng
;
Chen, You-Yin
;
Liu, Dean-Mo
In this study, a novel nanocarrier (MSN@Fe(3)O(4)) is constructed using a facile technology by capping mesoporous silica nanoparticles (MSN) with monodispersed Fe(3)O(4) nanoparticles through chemical bonding. The chemical links provide adhesion, which permits the magnetic nanoparticles, as nano-caps, to efficiently cover the mesoporous pores on the mesoporous silica matrix and be tightly bonded with the matrix surface. Without magnetic stimulus, none or only a negligible amount of the drug can be released from the MSN@Fe(3)O(4). However, when subjected to an external controllable magnetic field, a quantity of nano-caps can be remotely and precisely removed, giving tunable release profiles for an anticancer drug, (S)-(+)-camptothecin (CPT), with various dosages depending upon the strength and time period of magnetic induction. The transverse relaxivity (r(2)) of the MSN@Fe(3)O(4) nanocarriers was measured to be about 121.57 s(-1)mM(-1) Fe, which is larger than that for the reported mesoporous silica nanoparticles decorated with magnetite nanocrystals. Therefore, MSN@Fe(3)O(4) nanocarriers could perform well as T(2)-type MR contrast enhancement agents for cell or molecular imaging. In addition, the MSN@Fe(3)O(4) nanocarriers also demonstrate fairly high cell uptake efficiency. Together with its versatile magnetic manipulation, this new type of MSN@Fe(3)O(4) nanosystem can be considered as a new class of multifunctional nanodevice, with combined tunable drug release and nanoimaging modalities for a variety of biomedical uses.
Multifunctional magnetically removable nanogated lids of Fe3O4-capped mesoporous silica nanoparticles for intracellular controlled release and MR imaging
(2011-01-01)
Chen, Po-Jung
;
Hu, Shang-Hsiu
;
Hsiao, Chi-Sheng
;
Chen, You-Yin
;
Liu, Dean-Mo
In this study, a novel nanocarrier (MSN@Fe3O4) is constructed using a facile technology by capping mesoporous silica nanoparticles (MSN) with monodispersed Fe3O4 nanoparticles through chemical bonding. The chemical links provide adhesion, which permits the magnetic nanoparticles, as nano-caps, to efficiently cover the mesoporous pores on the mesoporous silica matrix and be tightly bonded with the matrix surface. Without magnetic stimulus, none or only a negligible amount of the drug can be released from the MSN@Fe3O4. However, when subjected to an external controllable magnetic field, a quantity of nano-caps can be remotely and precisely removed, giving tunable release profiles for an anticancer drug, (S)-(+)-camptothecin (CPT), with various dosages depending upon the strength and time period of magnetic induction. The transverse relaxivity (r(2)) of the MSN@Fe3O4 nanocarriers was measured to be about 121.57 s(-1)mM(-1) Fe, which is larger than that for the reported mesoporous silica nanoparticles decorated with magnetite nanocrystals. Therefore, MSN@Fe3O4 nanocarriers could perform well as T-2-type MR contrast enhancement agents for cell or molecular imaging. In addition, the MSN@Fe3O4 nanocarriers also demonstrate fairly high cell uptake efficiency. Together with its versatile magnetic manipulation, this new type of MSN@Fe3O4 nanosystem can be considered as a new class of multifunctional nanodevice, with combined tunable drug release and nanoimaging modalities for a variety of biomedical uses.
Multitheragnostic Multi-GNRs Crystal-Seeded Magnetic Nanoseaurchin for Enhanced In Vivo Mesenchymal-Stem-Cell Homing, Multimodal Imaging, and Stroke Therapy
(2015-11-04)
Chen, Po-Jung
;
Kang, Yi-Da
;
Lin, Chen-Huan
;
Chen, San-Yuan
;
Hsieh, Chia-Hung
A multifunctional nanoseaurchin probe in which mesoporous silica nanobeads with iron oxide nanoparticles embedded and multi-gold nanorods crystal-seeded are fabricated and labeled with umbilical cord mesenchymal stem cells through endocytosis. This nanoplatform enables efficient magnetic remote-controlled guiding for stem cell homing, and provides dual modalities of photoacoustic imaging and magnetic resonance imaging for in situ tracking and long-term monitoring to achieve therapeutic efficacy.
NIR-Triggered Synergic Photo-chemothermal Therapy Delivered by Reduced Graphene Oxide/Carbon/Mesoporous Silica Nanocookies
(2014-01-01)
Chen, Yu-Wei
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Chen, Po-Jung
;
Hu, Shang-Hsiu
;
Chen, I-Wei
;
Chen, San-Yuan
Surfactant-Free, Lipo-Polymersomes Stabilized by Iron Oxide Nanoparticles/Polymer Interlayer for Synergistically Targeted and Magnetically Guided Gene Delivery
(2014-02-01)
Hu, Shang-Hsiu
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Hsieh, Tsung-Ying
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Chiang, Chin-Sheng
;
Chen, Po-Jung
;
Chen, You-Yin
Gene therapy holds promise to suppress carcinomas but still remains far removed from clinic because of the lack of a safe and effective delivery system. Besides enhancing transfection efficiency, the difficulty in gene therapy is how to deliver sufficient gene molecules to the site of interest. Herein, the rational design of surfactant-free lipo-polymersomes (LPPs) to overcome these problems is reported, simultaneously using a lipid-stabilized double emulsion approach. The LPPs are designed to conceal the cationic lipids and plasmid DNA inside the core along with iron oxide nanoparticles/polymer interlayer and a relatively neutral lipid shell to avoid the undesired interaction during circulation, leading to high accumulation in the tumors of mice. Furthermore, guided by an external magnetic field and the folic acid (FA) that target tumors via folate receptor-mediated endocytosis on the cell surface, the vectors demonstrate a 30-40-fold increase in cell uptake. Moreover, this synergistic tumor-targeted approach can enhance a 10-fold increase in in vivo transfection efficacy by promoting the delivery of LPPs to cancer cells and facilitating the endosomal escape of gene molecules. The new insights and capabilities represent a major step in nanovector engineering for safe and efficient gene delivery.