Browsing by Author "Chen, Shin-Yu"

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FEATURE-BASED IMAGE REGISTRATION OF ALOS PALSAR AND AVNIR-2 IMAGES
(2011)
Teo, Tee-Ann
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Chen, Shin-Yu
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土木工程學系
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Department of Civil Engineering
The integration of optical and radar sensors in a common area is an important task in data fusion as well as other applications. The objective of this study is to perform the feature-based registration of ALOS PALSAR and AVNIR-2 images. The proposed method iteratively minimizes the discrepancy between the feature edges in PALSAR and AVNIR-2 images. A coarse-to-fine strategy is also adopted by applying an image pyramid. The accuracy of feature-based matching has increased from low to high resolution. The test images are synchronous PALSAR and AVNIR-2 images in Japan. The experimental results indicate that one pixel accuracy can be achieved using feature-based matching.
Identification of Protein Domains on Major Pilin MrkA That Affects the Mechanical Properties of Klebsiella pneumoniae Type 3 Fimbriae
(2012-05-15)
Chan, Chia-Han
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Chen, Feng-Jung
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Huang, Ying-Jung
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Chen, Shin-Yu
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Liu, Kuo-Liang
The Klebsiella pneumoniae type 3 fimbriae are mainly composed of MrkA pilins that assemble into a helixlike filament. This study determined the biomechanical properties of the fimbriae and analyzed 11 site-directed MrkA mutants to identify domains that are critical for the properties. Escherichia coli strains expressing type 3 fimbriae with an Ala substitution at either F34, V45, C87, G189, T196, or Y197 resulted in a significant reduction in biofilm formation. The E. coli strain expressing MrkAG189A remained capable of producing a normal number of fimbriae. Although F34A, V45A, T196A, and Y197A substitutions expressed on E. coli strains produced sparse quantities of fimbriae, no fimbriae were observed on the cells expressing MrkAC87A. Further investigations of the mechanical properties of the MrkAG189A fimbriae with optical tweezers revealed that, unlike the wild-type fimbriae, the uncoiling force for MrkAG189A fimbriae was not constant. The MrkAG189A fimbriae also exhibited a lower enthalpy in the differential scanning calorimetry analysis. Together, these findings indicate that the mutant fimbriae are less stable than the wildtype. This study has demonstrated that the C-terminal beta strands of MrkA are required for the assembly and structural stability of fimbriae.
Real-Time Fab-Wise Airborne Molecular Contaminant (AMC) Monitoring System Using Multiple Fourier Transform Infrared (FTIR) Spectrometers in a Semiconductor Plant
(2015-08-01)
Tsao, Yung-Chieh
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Chang, Pao-Erh
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Chen, Shin-Yu
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Hwang, Yaw-Huei
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環境工程研究所
The objective of this study was to investigate the airborne pollutant emission sources and fluctuations around the indoor and outdoor environments of a semiconductor manufacturing plant using monitoring data that were collected over 4 consecutive days via three Fourier transform infrared (FTIR) spectrometers located near an outdoor make-up air unit and an indoor Fab and sub Fab. Based on a total of 1,032 five-minute-interval records, fourteen chemicals were detected. Six of these chemicals, namely, carbon tetrafluoride, nitrous oxide, carbon monoxide, silane, sulfur hexafluoride, and methane, had significant concentration correlations between the indoor and outdoor environments. With the exception of silane and sulfur hexafluoride, the percentage of indoor/outdoor concentration ratios that were greater than one ranged from 62.2% to 73.1%, indicating that the indoor chemical concentrations were typically higher than the outdoor concentrations. Based on the regression models derived for the indoor and outdoor nitrous oxide concentrations, the nitrous oxide was believed to be originally emitted from the sub Fab vented to the outdoors and then partially returned to the Fab. It was estimated that for one ppb of nitrous oxide detected in the Fab, 2.58 ppb of nitrous oxide could be detected at the make-up air unit, which might result from the sub Fab emission being at a high level of 6.60 ppb. Furthermore, elevated outdoor concentrations of chemicals, such as carbon tetrafluoride, nitrous oxide and carbon monoxide, were observed without previous indoor emission peaks, indicating that these chemicals might accumulate in the outdoor surrounding area. This study successfully illustrated the dynamically changing relationship between indoor and outdoor chemical concentrations in a semiconductor clean room. These results can be used to prevent subtle and potential adverse impacts of airborne molecular contaminant (AMC) in various manufacturing facilities of technological industries, including the semiconductor and optoelectronics industries.