Browsing by Author "Chen, Shiou-Shiou"

Now showing 1 - 2 of 2

Nanoarchitectured structure and population dynamics of anaerobic ammonium oxidizing (anammox) bacteria in a wastewater treatment plant
(2020-09-05)
Huang, Yu-Tzu
;
Chen, Shiou-Shiou
;
Jetten, Mike S. M.
;
Lin, Jih-Gaw
;
環境工程研究所
Studies on microbial community and population dynamics are essential for the successful development, monitoring and operation of biological wastewater treatment systems. Especially for novel or sustainable systems such as the anaerobic ammonium oxidizing (anammox) process that are not yet well explored. Here we collected granular microbial sludge samples and investigated a community of anammox bacteria over a period of four years, divided into eight stages in a full scale simultaneous partial nitrification, anammox and denitrification (SNAD) process for treating landfill leachate. Specific qPCR primers were designed to target and quantify the two most abundant anammox species, Candidatus Kuenenia stuttgartiensis (KS) and Candidatus Brocadia anammoxidans (BA). The two species were monitored and could explain the dynamic shift of the anammox community corresponding to the operating conditions. Using the newly designed KS-specific primer (KSqF3/KSqR3) and BA-specific primer (BAqF/BAqR), we estimated the amounts of KS and BA to be in the range of 6.2 x 10(6) to 5.9 x 10(8) and 1.1 x 10(5) to 4.1 x 10(7) copies mu g(-1) DNA, respectively. KS was found to be the dominant species in all anammox granules studied and played an important role in the formation of granules. The KS/BA ratio was positively correlated to the size of granules in the reactor and ammonia nitrogen removal efficiency of the treatment plant.
Partial nitrification and anammox process: A method for high strength optoelectronic industrial wastewater treatment
(2013-06-01)
Daverey, Achlesh
;
Su, Sin-Han
;
Huang, Yu-Tzu
;
Chen, Shiou-Shiou
;
Sung, Shihwu
Completely autotrophic nitrogen removal over nitrite (CANON) process was employed in an 18 L sequencing batch reactor (SBR) for treatment of optoelectronic industrial wastewater containing high strength ammonium nitrogen (3712 +/- 120 mg NH4+ - N L-1). About 89% of total nitrogen and 98% of NH4+ - N removal efficiencies were observed at the loading rate of 909 g N m(-3) d(-1) and the HRT of 4 d. A profound variation in the performance of CANON process was experienced at high DO exposure (above 1 mg L-1) and high nitrite concentration (above 100 mg L-1). Inhibition due to high DO exposure was found to be reversible phenomenon whereas the synergistic inhibition of nitrite, free ammonia and free nitrous acid was irreversible. The fluctuation of reactor temperature between 17 and 37 degrees C did not affect the performance of CANON system. The CANON process was stably controlled at high nitrogen loading rate for more than one month. The co-existence of aerobic and anaerobic ammonium oxidizing bacteria in the reactor was detected by The PCR analysis. About 5 fold increase in amount of anammox bacteria over a period of 258 days was confirmed from the results of qPCR on day 487. (C) 2013 Elsevier Ltd. All rights reserved.