Browsing by Author "Chen, Tsai-Yu"

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Cultivation of Chlorella sp GD using piggery wastewater for biomass and lipid production
(2015-10-01)
Kuo, Chiu-Mei
;
Chen, Tsai-Yu
;
Lin, Tsung-Hsien
;
Kao, Chien-Ya
;
Lai, Jinn-Tsyy
The development of a culture system for Chlorella sp. GD to efficiently produce biomass and oil for biodiesel production was investigated. Chlorella sp. GD was cultivated with 0%, 25%, 50%, 75% and 100% piggery wastewater (diluted by medium) at 300 mu mol m(-2) s(-1), a 2% CO2 aeration rate of 0.2 vvm and 26 +/- 1 degrees C; after a 10-day culture in batch cultures, the maximum specific growth rate and biomass productivity of the microalga obtained in 100% piggery wastewater were 0.839 d(-1) and 0.681 g L-1 d(-1), respectively. The highest lipid content and lipid productivity were 29.3% and 0.155 g L-1 d(-1) at 25% wastewater, respectively. In semi-continuous cultures, the biomass and lipid productivities with 25-75% wastewater ratios were greater than 0.852 and 0.128 g L-1 d(-1), respectively. These results show that Chlorella sp. GD grows efficiently in piggery wastewater, and that a stable growth performance was achieved for long-term microalgal cultivation in a semi-continuous culture. (C) 2015 Elsevier Ltd. All rights reserved.
Cultivation of microalgal Chlorella for biomass and lipid production using wastewater as nutrient resource
(2015-05-01)
Chiu, Sheng-Yi
;
Kao, Chien-Ya
;
Chen, Tsai-Yu
;
Chang, Yu-Bin
;
Kuo, Chiu-Mei
Using wastewater for microalgal cultures is beneficial for minimizing the use of freshwater, reducing the cost of nutrient addition, removing nitrogen and phosphorus from wastewater and producing microalgal biomass as bioresources for biofuel or high-value by-products. There are three main sources of wastewater, municipal (domestic), agricultural and industrial wastewater, which contain a variety of ingredients. Some components in the wastewater, such as nitrogen and phosphorus, are useful ingredients for microalgal cultures. In this review, the effects on the biomass and lipid production of microalgal Chlorella cultures using different kinds of wastewater were summarized. The use of the nutrients resource in wastewater for microalgal cultures was also reviewed. The effect of ammonium in wastewater on microalgal Chlorella growth was intensively discussed. In the end, limitations of wastewater-based of microalgal culture were commented in this review article. (C) 2014 Elsevier Ltd. All rights reserved.
Ferrofluid-assisted rapid and directional harvesting of marine microalgal Chlorella sp used for biodiesel production
(2017-11-01)
Ho, Shih-Hsin
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Chiu, Sheng-Yi
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Kao, Chien-Ya
;
Chen, Tsai-Yu
;
Chang, Yu-Bin
In this work, a novel harvesting strategy using ferrofluids coupled with flocculation as a magnetic directional harvesting system was developed, providing a fast and easy way to effectively collect microalgae with no further modifications made to the ferrofluids. With a ferrofluid dosage of 25 mg L-1, a high harvesting efficiency of 95-100% was achieved within 1 min. In addition, we successfully performed a wastewater recycling strategy coupled with a microalgal ferrofluid-harvesting dynamic flow-through system to harvest biomass of Chlorella sp. MTF-7 which could achieve over 80% of the maximum level after three repeated recycling cultivations. This work demonstrated the use of an integrated microalgal ferrofluid-harvesting dynamic flow-through system to develop a simple and effective strategy to enhance microalgal harvesting efficiency, along with wastewater recycling, in a marine microalgal Chlorella sp. MTF-7. (C) 2017 Elsevier Ltd. All rights reserved.
Utilization of carbon dioxide in industrial flue gases for the cultivation of microalga Chlorella sp.
(2014-08-01)
Kao, Chien-Ya
;
Chen, Tsai-Yu
;
Chang, Yu-Bin
;
Chiu, Tzai-Wen
;
Lin, Hsiun-Yu
The biomass and lipid productivity of Chlorella sp. MTF-15 cultivated using aeration with flue gases from a coke oven, hot stove or power plant in a steel plant of the China Steel Corporation in Taiwan were investigated. Using the flue gas from the coke oven, hot stove or power plant for cultivation, the microalgal strain obtained a maximum specific growth rate and lipid production of (0.827 d(-1), 0.688 g L-1), (0.762 d(-1), 0.961 g L-1), and (0.728 d(-1), 0.792 g L-1), respectively. This study demonstrated that Chlorella sp. MTF-15 could efficiently utilize the CO2, NOX and SO2 present in the different flue gases. The results also showed that the growth potential, lipid production and fatty acid composition of the microalgal strain were dependent on the composition of the flue gas and on the operating strategy deployed. (C) 2014 Elsevier Ltd. All rights reserved.