Browsing by Author "Chen, Yi-Hsiang"

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Novel Role for miR-1290 in Host Species Specificity of Influenza A Virus
(2019-09-06)
Huang, Sheng-Yu
;
Huang, Chih-Heng
;
Chen, Chi-Jene
;
Chen, Ting-Wen
;
Lin, Chun-Yuan
The role of microRNA (miRNA) in influenza A virus (IAV) host species specificity is not well understood as yet. Here, we show that a host miRNA, miR-1290, is induced through the extracellular signal-regulated kinase (ERK) pathway upon IAV infection and is associated with increased viral titers in human cells and ferret animal models. miR-1290 was observed to target and reduce expression of the host vimentin gene. Vimentin binds with the PB2 subunit of influenza A virus ribonucleoprotein (vRNP), and knockdown of vimentin expression significantly increased vRNP nuclear retention and viral polymerase activity. Interestingly, miR-1290 was not detected in either chicken cells or mouse animal models, and the 3' UTR of the chicken vimentin gene contains no binding site for miR-1290. These findings point to a host species-specific mechanism by which IAV upregulates miR-1290 to disrupt vimentin expression and retain vRNP in the nucleus, thereby enhancing viral polymerase activity and viral replication.
One-Pot Synthesis of Highly Substituted Polyheteroaromatic Compounds by Rhodium(III)-Catalyzed Multiple C-H Activation and Annulation
(2014-09-08)
Jayakumar, Jayachandran
;
Parthasarathy, Kanniyappan
;
Chen, Yi-Hsiang
;
Lee, Tai-Hua
;
Chuang, Shih-Ching
A new method for the synthesis of highly substituted naphthyridine-based polyheteroaromatic compounds in high yields proceeds through rhodium(III)-catalyzed multiple CH bond cleavage and CC and CN bond formation in a one-pot process. Such highly substituted polyheteroaromatic compounds have attracted much attention because of their unique -conjugation, which make them suitable materials for organic semiconductors and luminescent materials. Furthermore, a possible mechanism, which involves multiple chelation-assisted ortho CH activation, alkyne insertion, and reductive elimination, is proposed for this transformation.