Browsing by Author "Cheng, Ya-Wen"

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Electromagnetic Coil/Inductance-Based Nondestructive Methods for Locating Distal Screw-Holes of an Intramedullary Interlocking-Nail
(2013)
Chung, Tien-Kan
;
Chu, Hou-Jen
;
Wong, Tze-Hong
;
Cheng, Ya-Wen
;
Hsu, Wensyang
We report an electromagnetic inductance/coil-based non-destructive method to target distal screw-holes in an intramedullary interlocking-nail surgical operation for fixing a long-bone fracture. The method is a radiation-free approach addressing the over-exposure issue of radioactivity caused by the typical X-ray-imaging approach. According to the method, we fabricate a targeting-system consisting of an internal inductance, external coil, guiding-mechanism, and driving/measurement electronics. When a voltage is applied to the internal inductance embedded in one of the distal screw-holes of a nail inserted in a bone, a directional magnetic flux is generated by the internal inductance due to the electromagnetic induction. Subsequently, the directional magnetic flux penetrates the nail and bone. When the external coil outside the bone scans along the axial and angular directions of the nail/bone, different amount of the generated magnetic flux is detected by the coil and consequently corresponding voltage response is induced in the coil due to the electromagnetic induction. In contrast to the magnetic flux generated and detected by the inductance and coil, respectively, we also investigate the reverse physics-behavior of the flux transmission (i.e., flux generated and detected by the coil and inductance) in order to improve the approach. Finally, by correlating the induced-voltage responses with the scanned axial-locations along the nail/bone, correlation curves are plotted. Through analyzing the curves, a criterion for predicting the location of the screw-holes of the nail is established. When compared the predicted location with the actual location of the screw-hole, the maximum targeting error is 2 mm for locating a screw-hole with a diameter of 5 mm. The result shows the targeting-method is accurate, fast, and easy for the surgeons and significantly simplifies the existed interlocking-nail surgical procedures.
Expression Status of Ribonucleotide Reductase Small Subunits hRRM2/p53R2 as Prognostic Biomarkers in Stage I and II Non-small Cell Lung Cancer
(2011-10-01)
Hsu, Nan-Yung
;
Wu, Jeng-Yuan
;
Liu, Xiyong
;
Yen, Yun
;
Chen, Chih-Yi
Overexpression of ribonucleotide reductase M2 (hRRM2) and p53-dependent RR small subunit (p53R2) has been correlated with tumor malignancy and progression in several types of cancer. The aim of this study was to determine the association of p53R2/hRRM2 expression with clinicopathological characteristics of stage I and II non-small cell lung cancer (NSCLC). Immunohistochemistry was conducted on a tissue array that included 92 samples. Correlations between hRRM2 and p53R2 expression and clinicopathological factors, recurrence/metastasis, and outcomes were analyzed. The analyses revealed that there was no correlation between p53R2 expression and clinicopathological factors; hRRM2 was only positively related to poor tumor differentiation (p=0.006). Regarding overall survival during the follow-up period, patients with p53R2+/hRRM2- tumors had the best outcomes (p<0.01). Multivariant Cox analysis revealed that p53R2 (risk=0.232, 95% CI=0.086-0.626, p=0.004) not only served as a prognostic biomarker to predict survival, but also as an independent biomarker to predict disease-free survival (risk=0.545, 95% CI=0.301-0.987, p=0.045) of patients with NSCLC. Therefore, we consider that the expression of p53R2 can be used not only as a biomarker for overall survival, but also as an indicator for tumor recurrence. Based on our finding, p53R2 expression seems more important than that of hRRM2 in prognosis of early-stage lung cancer.
FINITE ELEMENT MODELING OF ELECTROMAGNETIC-COILS TARGETING SYSTEM FOR LOCATING DISTAL SCREW-HOLE IN INTRAMEDULLARY INTERLOCKING-NAIL SURGERY
(2018-01-01)
Cheng, Ya-Wen
;
Chen, Po-Wen
;
Wong, Tze-Hong
;
Chen, Chin-Chung
;
Hsu, Wensyang
In this paper, we report a finite element modeling of an electromagnetic-coils targeting system to locate a distal screw hole in intramedullary interlocking-nail surgery. The system consists of an internal coil, external coil, and control/measurement electronics. The internal coil is embedded in a distal screw-hole of the nail inserted into the bone. A current is applied to the internal coil to produce a directional magnetic flux penetrating the nail/bone. Thus, the external coil scans different regions of the nail/bone will receive different amount of magnetic flux, and thereby produces different voltage outputs due to the electromagnetic induction. By analyzing the voltage outputs, the distal screw-hole is targeted. However, to precisely apply this system to many different nails for people in different regions, modification and optimization of the system are needed. For rapid modification and optimization, we implement finite element method to model the targeting system. Because the modeling results are qualitatively consistent to experimental results, the modeling is successfully validated to be able to qualitatively predict experimental trends and thereby can be used for rapid system modification and optimization.
The APE1 Asp/Asp Genotype and the Combination of APE1 Asp/Asp and hOGG1-Cys Variants Are Associated With Increased p53 Mutation in Non-Small Cell Lung Cancer
(2012-11-01)
Lin, Chun-Hsuan
;
Chen, Po-Ming
;
Cheng, Ya-Wen
;
Chen, Chih-Yi
;
Yuan, Chiun-Jye
Background: The hOGG1 Ser326Cys polymorphism is associated with lung cancer risk, but there are limited data regarding an association between the APE1 Asp148Glu polymorphism and lung cancer. Biological evidence shows that the hOGG1-Cys allele results in less DNA repair activity; however, this is not associated with p53 mutation in lung cancer. Therefore, we investigated whether an interaction between 110001 and APE1 is associated with the frequency of p53 mutation in lung cancer. Methods: We studied 217 Taiwanese adults with primary lung cancer. DNA polymorphisms of hOGG1 and APE1 were determined by polymerase chain reaction (PCR)-based restriction fragment length polymorphism. Mutations in p53 exons 5-8 were detected by direct sequencing. Multiple logistic regression was used to estimate odds ratios (ORs) and 95% CIs for the risk of p53 mutation associated with polymorphisms of hOGG1 and APE I in lung cancer. Results: As expected, no association between hOGG1 polymorphism and p53 mutation was observed in this population. However, a higher risk of p53 mutation was found in participants with the APE I Asp/Asp genotype than in those with the APE1-Glu allele (OR, 2.15; 95% CI, 1.19-3.87; P = 0.011). The risk of p53 mutation was also higher in participants with APEI Asp/Asp plus hOGG1-Cys than in those with APE1-Glu plus hOGG1 Ser/Ser (OR, 3.72; 95% Cl, 1.33-10.40; P = 0.012). Conclusions: These results suggest that the APE I Asp/Asp genotype and the combination of the APE I Asp/Asp and hOGG1-Cys variants are associated with increased risk of p53 mutation in non small cell lung cancer.
Topographical control of cell-cell interaction in C6 glioma by nanodot arrays
(2014-05-21)
Lee, Chia-Hui
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Cheng, Ya-Wen
;
Huang, G. Steven
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材料科學與工程學系
;
分子醫學與生物工程研究所
Nanotopography modulates the physiological behavior of cells and cell-cell interactions, but the manner of communication remains unclear. Cell networking ( syncytium) of astroglia provides the optimal microenvironment for communication of the nervous system. C6 glioma cells were seeded on nanodot arrays with dot diameters ranging from 10 to 200 nm. Cell viability, morphology, cytoskeleton, and adhesion showed optimal cell growth on 50-nm nanodots if sufficient incubation was allowed. In particular, the astrocytic syncytium level maximized at 50 nm. The gap junction protein Cx43 showed size-dependent and time-dependent transport from the nucleus to the cell membrane. The transport efficiency was greatly enhanced by incubation on 50-nm nanodots. In summary, nanotopography is capable of modulating cell behavior and influencing the cell-cell interactions of astrocytes. By fine-tuning the nanoenvironment, it may be possible to regulate cell-cell communications and optimize the biocompatibility of neural implants.