Browsing by Author "Chia-Ching Chang"

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RECONSTITUTED APOLIPOPROTEIN B LIPOPARTICLE, A PREPARATION METHOD AND USES THEREOF
(2017-07-27)
Chia-Ching Chang
;
Gong-Shen Chen
;
Tsai-Mu Cheng
;
Hsueh-Liang Chu
The present invention provides a method for preparing a reconstituted apolipoprotein B lipoparticle and the method comprises steps of (a) dissolving an apolipoprotein B and a lipid in a first buffer containing 2 M to 8 M urea and 1 wt % to 15 wt % amphiphilic compounds to form a mixture; and (b) dialyzing the mixture against a second buffer containing 0 M to 2M urea and 0 wt % to 0.5 wt % amphiphilic compounds for 1 to several times for lowering concentrations of the urea and the amphiphilic compounds in the mixture. The present invention further provides an apolipoprotein B lipoparticle and a use for the production of an apolipoprotein B lipoparticle used for delivering a hydrophobic substance.