Browsing by Author "Chiang, Hsiang-Ling"

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Molecular recognition between insulin and dextran encapsulated gold nanoparticles
(2016-11)
Lee, Kai-Chieh
;
Chiang, Hsiang-Ling
;
Chiu, Wei-Ru
;
Chen, Yu-Chie
;
應用化學系
Insulin is a peptide hormone that can regulate the metabolism of carbohydrates and lipids. This hormone is closely related to glucose-uptake in cells and can control blood glucose levels. Dextran is a polysaccharide composed of glucose units. In this study, we discovered that dextran-encapsulated gold nanoparticles (AuNPs@Dextran) and nanoclusters (AuNCs@Dextran) can be used to recognize insulin. The dissociation constant of insulin toward AuNPs@Dextran was estimated to be approximate to 5.3x10(-6)M. The binding site on insulin toward the dextran on the nanoprobes was explored as well. It was found that the sequence of numbers 1-22 on the insulin B chain can interact with the dextran encapsulated nanoprobes. Additionally, we also demonstrated that the dextran-encapsulated nanoprobes could be used as concentration probes to selectively enrich trace amounts of insulin (approximate to 1pM) from serum samples. Copyright (c) 2016 John Wiley & Sons, Ltd.