Browsing by Author "Chiang, Ming-Fu"

Now showing 1 - 2 of 2

Biomechanical comparison of instrumented posterior lumbar interbody fusion with one or two cages by finite element analysis
(2006-09-01)
Chiang, Ming-Fu
;
Zhong, Zheng-Cheng
;
Chen, Chen-Sheng
;
Cheng, Cheng-Kung
;
Shih, Shih-Liang
Study Design. Using finite element models to study the biomechanics of lumbar instrumented posterior lumbar interbody fusion (PLIF) with one or two cages. Objective. Analyzing the biomechanics of instrumented PLIF with one or two cages as to evaluate whether a single cage is adequate for instrumented PLIF. Summary of Background Data. Implantation of a single cage in instrumented PLIF of lumbar spine is still controversial. Methods. Three validated finite element models of L3-L5 lumbar segment were established [intact model (INT), one cage model (LS-1), and two cages model (LS-2)]. The available finite element program ANSYS 6.0 (Swanson Analysis System Inc., Houston, TX) was applied. To analyze the biomechanics of these models, 10 Nm flexion, extension, rotation, and lateral bending moment with 150 N of preload were respectively imposed on the superior surfaces of the L3. Results. Compared with the INT model, the decrease of ROM in the LS-1 and LS-2 models were exaggerated from 0.67 degrees to 3.73 degrees and ranged from 37.2% to 86.1% in all motions. The mean subsidence was found to be slightly higher in the LS-1 model. Most of the cage dislodgement in both models was less than 0.03 mm. The mean dislodgement was slightly higher in the LS-1 model. The stress of cage was found to be high in the LS-2 model. The mean stress of screw was raised to 4.5% to 9.7% in the LS-1, which was higher than that in the LS-2 model. In general, stress of adjacent disc was more pronounced in the LS-2 model. The most stress distributed at the anterior portion of the adjacent disc, which could be used to interpret the clinical findings of the early adjacent disc degeneration. Conclusions. A single cage inserted in an instrumented PLIF gains approximate biomechanical stability, slight greater subsidence, and a slight increase in screw stress but less early degeneration in adjacent disc. Adjusting these factors, instrumented PLIF with one cage could be encouraged in clinical practice.
CNS-targeted AAV5 gene transfer results in global dispersal of vector and prevention of morphological and function deterioration in CNS of globoid cell leukodystrophy mouse model
(2011-08-01)
Lin, Dar-Shong
;
Hsiao, Chung-Der
;
Liau, Ian
;
Lin, Shuan-Pei
;
Chiang, Ming-Fu
Globoid cell leukodystrophy (GLD) is a devastating lysosomal storage disease caused by deficiency of the enzyme galactocerebrosidase (GALC). Currently, there is no definite cure for GLD. Several attempts with CNS-directed gene therapy in twitcher mice (a murine model of GLD) demonstrated restricted expression of GALC activity in CNS and failure of therapeutic efficacy in cerebellum and spinal cord, resulting in various degrees of correction of biochemical, pathological and clinical phenotype. More recently, twitcher mice receiving a combination of hematopoietic and viral vector gene transfer therapies were not protected from neurodegeneration and axonopathy in both cerebellum and spinal cord. This evidence indicates the requirement of sufficient and widespread GALC expression in CNS and rescue of cerebellum and spinal cord in the therapeutic intervention of murine model of GLD. In this study, we have optimized intracranial delivery of AAV2/5-GALC to the neocortex, hippocampus and cerebellum, instead of the thalamus as was previously conducted, of twitcher mice. The CNS-targeted AAV2/5 gene transfer effectively dispersed GALC transgene along the neuraxis of CNS as far as the lumbar spinal cord, and reduced the accumulation of psychosine in the CNS of twitcher mice. Most importantly, the treated twitcher mice were protected from loss of oligodendrocytes and Purkinje cells, axonopathy and marked gliosis, and had significantly improved neuromotor function and prolonged lifespan. These preclinical findings with our approach are encouraging, although a more robust response in the spinal cord would be desirable. Collectively, the information in this study validates the efficacy of this gene delivery approach to correct enzymatic deficiency, psychosine accumulation and neuropathy in CNS of GLD. Combining cell therapy such as bone marrow transplantation with treatment with the aim of reducing inflammation, replacing dead or dying oligodendrocytes and targeting PNS may provide a synergistic and more complete correction of this disease. (C) 2011 Elsevier Inc. All rights reserved.