Browsing by Author "Chiang, Shu-Jen"

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Multiphoton and confocal imaging of precisely cut oral cancer tumor sections for personalized medicine
(2020-01-01)
Chiang, Shu-Jen
;
Tseng, Po-Hang
;
Huang, Hsu-Cheng
;
Chen, Shi-Chi
;
Chen, Shean-Jen
Traditionally, testing of therapeutic agents uses two-dimensional cell cultures which does not recapitulate the complex, three-dimensional architecture of tissues. In the case of cancer drug development, immunotherapy (IT) emerged as an effective treatment strategy for the patients who respond to therapy. However, the overall patient response rate for immunotherapy is around 25%. Therefore, there is a need to develop more effective drug testing platform for cancer patients. In this work, we develop three-dimensional tissue cultures of human oral cancer slices. We observed the growth dynamics of the tissue sections for up to 28 days. Their viability over extended periods suggest that the tissue sections may be used for personalized drug testing.
Multiphoton imaging of dye penetration dynamics in tissue sections
(2020-01-01)
Tseng, Po-Hang
;
Chiang, Shu-Jen
;
Chen, Shean-Jen
;
Dong, Chen-Yuan
;
光電學院
Rapid and in-depth labeling biological tissue samples is invaluable for studying tissue organization in three-dimensions. The current strategy in finding optimal labeling parameters for dye molecules through tissues often requires trial and error testing. Difficulty in establishing a standard approach is due to lack of information on the diffusion parameters of dye through tissues. In this study, we investigate the temporal progression of dye penetration in different tissue types. By the combination of multiphoton imaging of labeled tissues and application of the theoretical model of diffusion with well-defined boundary conditions, we aim to arrive at systematic parameters for optimizing dye penetration. Determination of the effective diffusion coefficient of the dyes used will then be useful for further application.