Browsing by Author "Chien, Chih-Wei"

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An Informatics-assisted Label-free Approach for Personalized Tissue Membrane Proteomics: Case Study on Colorectal Cancer
(2011-04-01)
Han, Chia-Li
;
Chen, Jinn-Shiun
;
Chan, Err-Cheng
;
Wu, Chien-Peng
;
Yu, Kun-Hsing
We developed a multiplexed label-free quantification strategy, which integrates an efficient gel-assisted digestion protocol, high-performance liquid chromatography tandem MS analysis, and a bioinformatics alignment method to determine personalized proteomic profiles for membrane proteins in human tissues. This strategy provided accurate (6% error) and reproducible (34% relative S. D.) quantification of three independently purified membrane fractions from the same human colorectal cancer (CRC) tissue. Using CRC as a model, we constructed the personalized membrane protein atlas of paired tumor and adjacent normal tissues from 28 patients with different stages of CRC. Without fractionation, this strategy confidently quantified 856 proteins (>= 2 unique peptides) across different patients, including the first and robust detection (Mascot score: 22,074) of the well-documented CRC marker, carcinoembryonic antigen 5 by a discovery-type proteomics approach. Further validation of a panel of proteins, annexin A4, neutrophils defensin A1, and claudin 3, confirmed differential expression levels and high occurrences (48-70%) in 60 CRC patients. The most significant discovery is the overexpression of stomatin-like 2 (STOML2) for early diagnostic and prognostic potential. Increased expression of STOML2 was associated with decreased CRC-related survival; the mean survival period was 34.77 +/- 2.03 months in patients with high STOML2 expression, whereas 53.67 +/- 3.46 months was obtained for patients with low STOML2 expression. Further analysis by ELISA verified that plasma concentrations of STOML2 in early-stage CRC patients were elevated as compared with those of healthy individuals (p < 0.001), suggesting that STOML2 may be a noninvasive serological biomarker for early CRC diagnosis. The overall sensitivity of STOML2 for CRC detection was 71%, which increased to 87% when combined with CEA measurements. This study demonstrated a sensitive, label-free strategy for differential analysis of tissue membrane proteome, which may provide a roadmap for the subsequent identification of molecular target candidates of multiple cancer types. Molecular & Cellular Proteomics 10: 10.1074/mcp.M110.003087, 1-15, 2011.
Application of Ultrafast Laser Nanostructuring to Selective Laser Melting Parts
(2015-12)
Cheng, Chung-Wei
;
Chien, Chih-Wei
;
Lin, De-Yau
;
Horng, Ji-Bin
;
機械工程學系
A method is proposed for the fabrication of 3D metal (Ti6AL4V) parts that have microstructures covered with controllable nanostructures using selective laser melting (SLM) followed by laser-induced periodic surface structures (LIPSS). The metallic parts with microstructures was first fabricated by the SLM process, then a ultrafast laser machining platform was used to locally nanostructuring of the metal parts by LIPSS process. The experimental results show that periodic-like nanostructures with two different periods, i.e. 800-900 nm on the top surface and 450-600 nm on the bottom surface of the SLM-produced microstructures, were obtained simultaneously by a picosecond laser at wavelength 1064 mu with the same laser irradiation parameters. The liquid test demonstrates that the SLM+LIPSS process provides a structuring method to generate hydrophobic-enhanced metal parts. The process can provide a variety of 3D hierarchical micro/nanostructuring on metal material for biomedical application (e.g. customized implants) which is more than a conventional SLM process can provide.
Application of Ultrafast Laser Nanostructuring to Selective Laser Melting Parts
(2015-12-01)
Cheng, Chung-Wei
;
Chien, Chih-Wei
;
Lin, De-Yau
;
Horng, Ji-Bin
;
機械工程學系
A method is proposed for the fabrication of 3D metal (Ti6AL4V) parts that have microstructures covered with controllable nanostructures using selective laser melting (SLM) followed by laser-induced periodic surface structures (LIPSS). The metallic parts with microstructures was first fabricated by the SLM process, then a ultrafast laser machining platform was used to locally nanostructuring of the metal parts by LIPSS process. The experimental results show that periodic-like nanostructures with two different periods, i.e. 800-900 nm on the top surface and 450-600 nm on the bottom surface of the SLM-produced microstructures, were obtained simultaneously by a picosecond laser at wavelength 1064 mu with the same laser irradiation parameters. The liquid test demonstrates that the SLM+LIPSS process provides a structuring method to generate hydrophobic-enhanced metal parts. The process can provide a variety of 3D hierarchical micro/nanostructuring on metal material for biomedical application (e.g. customized implants) which is more than a conventional SLM process can provide.
Effects of Mechanical Strains on the Characteristics of Top-Gate Staggered a-IGZO Thin-Film Transistors Fabricated on Polyimide-Based Nanocomposite Substrates
(2012-07-01)
Lin, Chang-Yu
;
Chien, Chih-Wei
;
Wu, Chung-Chih
;
Yeh, Yung-Hui
;
Cheng, Chun-Cheng
In this paper, we had successfully implemented flexible top-gate staggered amorphous In-Ga-Zn-O (a-IGZO) thin-film transistors (TFTs) on colorless and transparent polyimide (PI)-based nanocomposite substrates using fully lithographic and etching processes that are compatible with existing TFT mass fabrication technologies. The use of the selectively coated release layer between the nanocomposite PI film and the glass carrier ensured smooth debonding of the plastic substrate after TFT fabrication. The TFTs showed decent performances (with mobility > 10 cm(2)/V . s) either as fabricated or as debonded from the carrier glass. By bending the devices to different radii of curvature (from a flat state to an outward bending radius of 5 mm), influences of mechanical strains on the characteristics of flexible a-IGZO TFTs were also investigated. In general, the mobility of the flexible a-IGZO TFT increased with the tensile strain, whereas the threshold voltage decreased with the tensile strain. The variation of the mobility in a-IGZO TFTs versus the strain appeared smaller than those observed for amorphous silicon TFTs.