Browsing by Author "Chiou, CC"

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Purification and characterization of neutral sphingomyelinase from Helicobacter pylori
(2000-04-25)
Chan, EC
;
Chang, CC
;
Li, YS
;
Chang, CA
;
Chiou, CC
Phospholipase activities of human gastric bacterium, Helicobacter pylori , are regarded as the pathogenic factors owing to their actions on epithelial eel membranes. In this study, we purified and characterized neutral sphingomyelinase (N-SMase) from the superficial components of H. pylori strains for the first time. N-SMase was purified 2083-fold with an overall recovery of 37%. The purification steps included acid glycine extraction, ammonium sulfate precipitation, CM-Sepharose, Mono-Q, and Sephadex G-75 column chromatography. Approximate molecular mass for the native N-SMase was around 32 kDa. When N-omega-trinitrophenylaminol auryl sphingomyelin (TNPAL-SM) was used as a substrate, the purified enzyme exhibited a K-m of 6.7 mu M and a V-max of 15.6 nmol of TNPAL-sphingosine/h/mg of protein at 37 degrees C in 50 mM phosphate-buffered saline, pH 7.4. N-SMase I-caches optimal activity at pH 7.4 and has a pi of 7.5. The enzyme activity is magnesium dependent and specifically hydrolyzed sphingomyelin and phosphatidylethanolamine. The enzyme also exhibits hemolytic activity on human erythrocytes. According to Western blot analysis, a rabbit antiserum against purified N-SMase from H. pylori cross-reacted with SMase from Bacillus cereus. Sera from individuals with H, pylori infection but not uninfected ones recognizing the purified N-SMase indicated that it was produced in vivo. In enzyme linked immunosorbent assays, the purified N-SMase used as an antigen was as effective as crude protein antigens in detecting human antibodies to H. pylori.
The design and implementation of the NCTUns 1.0 network simulator
(2003-06-05)
Wang, SY
;
Chou, CL
;
Huang, CH
;
Hwang, CC
;
Yang, ZM
This paper presents the design and implementation of the NCTUns 1.0 network simulator, which is a high-fidelity and extensible network simulator capable of simulating both wired and wireless IP networks. By using an enhanced simulation methodology, a new simulation engine architecture, and a distributed and open-system architecture, the NCTUns 1.0 network simulator is much more powerful than its predecessor-the Harvard network simulator, which was released to the public in 1999. The NCTUns 1.0 network simulator consists of many components. In this paper, we will present the design and implementation of these components and their interactions in detail. (C) 2003 Elsevier Science B.V. All rights reserved.