Browsing by Author "Chiu, Feng-Hsuan"
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Item type:Item, ORGN 609-Site-saturated mutagenesis of phenylalanine 445 of saccharomyces cerevisiae oxidosqualene-lanosterol cyclase results in production of isomalabaricane triterpenoid and parkeol(2006-09-10) ;Wu, Tung-Kung ;Liu, Yuan-Ting ;Chiu, Feng-Hsuan ;生物科技學系Department of Biological Science and TechnologyItem type:Item, Phenylalanine 445 within oxidosqualene-lanosterol cyclase from Saccharomyces cerevisiae influences C-ring cyclization and deprotonation reactions(2006-10-12) ;Wu, Tung-Kung ;Liu, Yuan-Ting ;Chiu, Feng-Hsuan ;Chang, Cheng-Hsiang生物科技學系We describe the Saccharomyces cerevisiae oxidosqualene - lanosterol cyclase Phe445 site-saturated mutants that generate truncated tricyclic and altered deprotonation product profiles. Among these mutants, only polar side-chain group substitutions genetically complemented yeast viability and produced spatially related product diversity, supporting the Johnson model that cation - pi interactions between a carbocationic intermediate and an enzyme can be replaced by an electrostatic or polar side chain to stabilize the cationic intermediate, but with product differentiation.