Browsing by Author "Chu, Chien-Ju"

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A fluorescent turn-on probe for detection of hypochlorus acid and its bioimaging in living cells
(2020-06-05)
Chu, Chien-Ju
;
Wu, Guan-Syuan
;
Ma, Ho-I
;
Venkatesan, Parthiban
;
Thirumalaivasan, Natesan
Hypochlorous acid has played several functions in the biological system. However, excess HOCI can cause damage to biomolecules and result in some diseases. Accordingly, a new fluorescent probe, BSP, has been developed for fast recognition of HOCI through the HOG-induced oxidation of methyl phenyl sulfide to sulfoxide. The reaction of BSP with HOG caused a 22-fold fluorescence enhancement (quantum yield increase from 0.006 to 0.133). The detection limit of HOCI is found to be 30 nM (S/N = 3). The fluorescence enhancement is due to the suppression of the photo-induced electron transfer from the methyl phenyl sulfide moiety to BODIPY. Eventually, the cellular fluorescence imaging experiment showed that BSP could be effectively used for monitoring HOCI in living cells. (C) 2020 Elsevier B.V. All rights reserved.
Zinc(II) and pyrophosphate selective fluorescence probe and its application to living cell imaging
(2015-02-01)
Lin, Jia-Rong
;
Chu, Chien-Ju
;
Venkatesan, Parthiban
;
Wu, Shu-Pao
;
應用化學系
A BODIPY-based fluorescent chemosensor 1 selectively detects Zn2+ by significant fluorescence enhancement. The binding ratio of the 1-Zn2+ complex was determined from a Job plot to be 1:1. The binding constant (K-a) of Zn2+ binding to chemosensor 1 was found to be 5.9 x 10(4) M-1, with a detection limit of 0.59 mu M. Furthermore, the 1-Zn2+ complex can specifically detect pyrophosphate (PPi) through fluorescence quenching. The detection limit for detecting PPi was found to be approximately 17.5 mu M. Fluorescence microscopy imaging using RAW264.7 cells showed that chemosensor 1 can be used as an effective fluorescent probe for detecting Zn2+ and PPi in living cells. (C) 2014 Elsevier B.V. All rights reserved.