Browsing by Author "Chu, Hsueh-Liang"

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Calcium dependent p21 (cip1/waf1) stabilizer cyclin I (Ccni)
(2008-04-01)
Chu, Hsueh-Liang
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Chen, Tzu-Hsuan
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Yeh, Yi-Chen
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Tsai, Chuan-Mei
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Wu, Chang-You
Development of a Growth- Hormone- Conjugated Nanodiamond Complex for Cancer Therapy
(2014-05-01)
Chu, Hsueh-Liang
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Chen, Hung-Wei
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Tseng, Shin-Hua
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Hsu, Ming-Hua
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Ho, Li-Ping
It is highly desirable to develop a therapeutic, observable nanoparticle complex for specific targeting in cancer therapy. Growth hormone (GH) and its antagonists have been explored as cancer cell-targeting molecules for both imaging and therapeutic applications. In this study, a low toxicity, biocompatible, therapeutic, and observable GH-nanoparticle complex for specifically targeting growth hormone receptor (GHR) in cancer cells was synthesized by conjugating GH with green fluorescence protein and carboxylated nanodiamond. Moreover, we have shown that this complex can be triggered by laser irradiation to create a nanoblast and induce cell death in the A549 non-small-cell lung cancer cell line via the apoptotic pathway. This laser-mediated, cancer-targeting platform can be widely used in cancer therapy.
Directly Thiolated Modification onto the Surface of Detonation Nanodiamonds
(2014-05-28)
Hsu, Ming-Hua
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Chuang, Hong
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Cheng, Fong-Yu
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Huang, Ying-Pei
;
Han, Chien-Chung
An efficient method for modifying the surface of detonation nanodiamonds (5 and 100 nm) with thiol groups (-SH) by using an organic chemistry strategy is presented herein. Thiolated nanodiamonds were characterized by spectroscopic techniques, and the atomic percentage of sulfur was analyzed by elemental analysis and X-ray photoelectron spectroscopy. The conjugation between thiolated nanodiamonds and gold nanoparticles was elucidated by transmission electron microscopy and UV-vis spectrometry. Moreover, the material did not show significant cytotoxicity to the human lung carcinoma cell line and may prospectively be applied in bioconjugated technology. The new method that we elucidated may significantly improve the approach to surface modification of detonation nanodiamonds and build up a new platform for the application of nanodiamonds.
Exploration and characterization of the memcapacitor and memristor properties of Ni-DNA nanowire devices
(2017-09-01)
Chu, Hsueh-Liang
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Lai, Jian-Jhong
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Wu, Li-Ying
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Chang, Shen-Lin
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Liu, Chia-Ming
A 2-mu m-long Ni ion-chelated DNA molecule (Ni-DNA) was found for the first time to possess both memcapacitor and memristor properties; this Ni-DNA molecule is known as a dual memory circuit element (memelement). As a memelement, the state of impedance on Ni-DNA is proportional to the unit number of Ni ions containing a base pair complex (Ni-bp), which is determined by the previously applied external voltage. Interestingly, the impedances of Ni-DNA change in response to a change in the sweeping frequencies of the external bias. Our simulation results also indicate that changes in the effective resistance and capacitance of Ni-bp may be attributed to changes in the Ni ion redox species in the Ni-bp of a Ni-DNA nanowire. Therefore, the working mechanism of a nanowire-type memcapacitor and memristor is revealed. In summary, the Ni-DNA nanowire is shown to be a multi-dimensional memory device, whose memory state depends on the length of DNA and applied external voltages/frequencies.
Folding and Characterization of Intrinsically Disordered Protein Human Cyclin I (Ccni)
(2012-01-31)
Chu, Hsueh-Liang
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Cheng, Tsai-Mu
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Li, Hsing-Yuan
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Chang, Chia-Ching
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交大名義發表
Human haptoglobin phenotypes and concentration determination by nanogold-enhanced electrochemical impedance spectroscopy
(2011-05-17)
Cheng, Tsai-Mu
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Lee, Tzu-Cheng
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Tseng, Shin-Hua
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Chu, Hsueh-Liang
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Pan, Ju-Pin
Haptoglobin (Hp) is an acute phase protein that binds free hemoglobin (Hb), preventing Hb-induced oxidative damage in the vascular system. There are three phenotypes in human Hp, whose heterogeneous polymorphic structures and varying concentrations in plasma have been attributed to the cause of diseases and outcome of clinical treatments. Different phenotypes of Hp may be composed of the same subunits but different copy numbers, rendering their determination difficult by a single procedure. In this study, we have developed a simple, fast, reliable and sensitive method, using label-free nanogold-modified bioprobes coupled with self-development electrochemical impedance spectroscopy (EIS). By this method, probe surface charge transfer resistance is detected. The relative charge transfer resistance ratios for Hp 1-1, Hp 2-1 and Hp 2-2 were characterized. We were able to determine protein size difference within 3 nm, and the linear region of the calibration curve for Hp levels in the range of 90 pg ml(-1) and 90 mu g ml(-1) (similar to 1 fM to 1 pM). We surmise that similar approaches can be used to investigate protein polymorphism and altered protein-protein interaction associated with diseases.
Human securin: a p53 mediated protooncogene
(2007-04-01)
Wu, Chang You
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Chao, Jui-I
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Chu, Hsueh-Liang
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Chang, Pei-Hsin
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Lo, Chiu-An
Laser Induced Popcorn-like Conformational Transition of Nano-diamond as a Nanoknife
(2009-02-01)
Chang, Chia-Ching
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Chu, Hsueh-Liang
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Lee, Tzu-Cheng
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Chou, Ching-Chung
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交大名義發表
Laser induced popcornlike conformational transition of nanodiamond as a nanoknife
(2008-07-21)
Chang, Chia-Ching
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Chen, Pei-Hsin
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Chu, Hsueh-Liang
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Lee, Tzu-Cheng
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Chou, Ching-Chung
Nanodiamond (ND) is surrounded by layers of graphite on its surface. This unique structure feature creates unusual fluorescence spectra, which can be used as an indicator to monitor its surface modification. Meanwhile, the impurity, nitroso (C - N = O) inside the ND can be photolyzed by two-photon absorption, releasing NO to facilitate the formation of a sp(3) diamond structure in the core of ND and transforming it into a sp(2) graphite structure. Such a conformational transition enlarges the size of ND from 8 to 90 nm, resulting in a popcornlike structure. This transition reaction may be useful as nanoknives in biomedical application. (c) 2008 American Institute of Physics.
Molecular Mechanism of Nanodiamonds Attacking Cancer Cell
(2010-04-01)
Chen, Hong-Wei
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Cheng, Tsai-Mu
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Chu, Hsueh-Liang
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Chang, Chia-Ching
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生物科技學系
Ni(2+)-Enhanced Charge Transport via pi-pi Stacking Corridor in Metallic DNA
(2011-02-16)
Tseng, Shin-Hua
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JangJian, Peng-Chung
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Tsai, Chuan-Mei
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Cheng, Tsai-Mu
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Chu, Hsueh-Liang
The mechanism underlying DNA charge transport is intriguing. However, poor conductivity of DNA makes it difficult to detect DNA charge transport. Metallic DNA (M-DNA) has better conducting properties than native DNA. Ni(2+) may chelate in DNA and thus enhance DNA conductivity. On the basis of this finding, it is possible to reveal the mechanisms underlying DNA charge transport. The conductivity of various Ni-DNA species such as single-stranded, full complement, or mismatched sequence molecules was systematically tested with ultraviolet absorption and electrical or chemical methods. The results showed that the conductivity of single-stranded Ni-DNA (Ni-ssDNA) was similar to that of a native DNA duplex. Moreover, the resistance of Ni-DNA with a single basepair mismatch was significantly higher than that of fully complementary Ni-DNA duplexes. The resistance also increased exponentially as the number of mismatched basepairs increased linearly after the tunneling current behavior predicted by the Simmons model. In conclusion, the charges in Ni(2+)-doped DNA are transported through the Ni(2+)-mediated pi-pi stacking corridor. Furthermore, Ni-DNA acts as a conducting wire and exhibits a tunneling barrier when basepair mismatches occur. This property may be useful in detecting single basepair mismatches.
Ni2+-Enhanced Charge Transport via pi-pi Stacking Corridor in Metallic DNA
(2011-02-16)
Tseng, Shin-Hua
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JangJian, Peng-Chung
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Tsai, Chuan-Mei
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Cheng, Tsai-Mu
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Chu, Hsueh-Liang
The mechanism underlying DNA charge transport is intriguing. However, poor conductivity of DNA makes it difficult to detect DNA charge transport. Metallic DNA (M-DNA) has better conducting properties than native DNA. Ni2+ may chelate in DNA and thus enhance DNA conductivity. On the basis of this finding, it is possible to reveal the mechanisms underlying DNA charge transport. The conductivity of various Ni-DNA species such as single-stranded, full complement, or mismatched sequence molecules was systematically tested with ultraviolet absorption and electrical or chemical methods. The results showed that the conductivity of single-stranded Ni-DNA (Ni-ssDNA) was similar to that of a native DNA duplex. Moreover, the resistance of Ni-DNA with a single basepair mismatch was significantly higher than that of fully complementary Ni-DNA duplexes. The resistance also increased exponentially as the number of mismatched basepairs increased linearly after the tunneling current behavior predicted by the Simmons model. In conclusion, the charges in Ni2+-doped DNA are transported through the Ni2+-mediated pi-pi stacking corridor. Furthermore, Ni-DNA acts as a conducting wire and exhibits a tunneling barrier when basepair mismatches occur. This property may be useful in detecting single basepair mismatches.
Programmable Redox State of the Nickel Ion Chain in DNA
(2014-02-01)
Chu, Hsueh-Liang
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Chiu, Shao-Chien
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Sung, Ching-Feng
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Tseng, Wellen
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Chang, Yu-Chuan
DNA is a nanowire in nature which chelates Ni ions and forms a conducting chain in its base-pairs (Ni-DNA). Each Ni ion in Ni-DNA exhibits low (Ni2+) or high (Ni3+) oxidation state and can be switched sequentially by applying bias voltage with different polarities and writing times. The ratio of low. and high oxidation states of Ni ions in Ni-DNA represents a programmable multistate memory system with an added capacitive component, in which multistate information can be written, read, and erased. This study also indicates that the biomolecule-based self-organized nanostructure can be used as a template for nanodevice fabrication.
Rapid and highly sensitive detection of Enterovirus 71 by using nanogold-enhanced electrochemical impedance spectroscopy
(2013-07-19)
Li, Hsing-Yuan
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Tseng, Shing-Hua
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Cheng, Tsai-Mu
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Chu, Hsueh-Liang
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Lu, Yu-Ning
Enterovirus 71 (EV71) infection is an emerging infectious disease causing neurological complications and/or death within two to three days after the development of fever and rash. A low viral titre in clinical specimens makes the detection of EV71 difficult. Conventional approaches for detecting EV71 are time consuming, poorly sensitive, or complicated, and cannot be used effectively for clinical diagnosis. Furthermore, EV71 and Coxsackie virus A16 (CA16) may cross react in conventional assays. Therefore, a rapid, highly sensitive, specific, and user-friendly test is needed. We developed an EV71-specific nanogold-modified working electrode for electrochemical impedance spectroscopy in the detection of EV71. Our results show that EV71 can be distinguished from CA16, Herpes simplex virus, and lysozyme, with the modified nanogold electrode being able to detect EV71 in concentrations as low as 1 copy number/50 mu 1 reaction volume, and the duration between sample preparation and detection being 11 min. This detection platform may have the potential for use in point-of-care diagnostics.
Rapid and highly sensitive detection of Enterovirus 71 by using nanogold-enhanced electrochemical impedance spectroscopy (vol 24, 285102, 2013)
(2013-10-04)
Li, Hsing-Yuan
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Tseng, Shing-Hua
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Cheng, Tsai-Mu
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Chu, Hsueh-Liang
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Lu, Yu-Ning
Refolding of ybeA: a native knotted protein
(2008-04-01)
Yeh, Yi-Chen
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Chen, Tzu-Hsuan
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Chu, Hsueh-Liang
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Tsai, Chuan-Mei
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Chang, Chia-Ching
Single molecular observation of the apolipoprotein B and its folding intermediates in reassembled low density lipoprotein
(2010-04-01)
Chang, Chia-Ching
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Chu, Hsueh-Liang
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Cheng, Tsai-Mu
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交大名義發表
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National Chiao Tung University
Structure and function analysis of new member of cyclin family: Cyclin I
(2007-04-01)
Chu, Hsueh-Liang
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Chao, Jui-I
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Wu, Chang-You
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Chang, Pei-Hsin
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Lo, Chiu-An
Surface electromyography analysis of blepharoptosis correction by transconjunctival incisions
(2016-06)
Tu, Lung-Chen
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Wu, Ming-Chya
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Chu, Hsueh-Liang
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Chiang, Yi-Pin
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Kuo, Chih-Lin
Upper eyelid movement depends on the antagonistic actions of orbicularis oculi muscle and levator aponeurosis. Blepharoptosis is an abnormal drooping of upper eyelid margin with the eye in primary position of gaze. Transconjunctival incisions for upper eyelid ptosis correction have been a welldeveloped technique. Conventional prognosis however depends on clinical observations and lacks of quantitatively analysis for the eyelid muscle controlling. This study examines the possibility of using the assessments of temporal correlation in surface electromyography (SEMG) as a quantitative description for the change of muscle controlling after operation. Eyelid SEMG was measured from patients with blepharoptosis preoperatively and postoperatively, as well as, for comparative study, from young and aged normal subjects. The data were analyzed using the detrended fluctuation analysis method. The results show that the temporal correlation of the SEMG signals can be characterized by two indices associated with the correlation properties in short and long time scales demarcated at 3 ms, corresponding to the time scale of neural response. Aging causes degradation of the correlation properties at both time scales, and patient group likely possess more serious correlation degradation in long-time regime which was improved moderately by the ptosis corrections. We propose that the temporal correlation in SEMG signals may be regarded as an indicator for evaluating the performance of eyelid muscle controlling in postoperative recovery. (C) 2016 Elsevier Ltd. All rights reserved.
Synthesis of Apolipoprotein B Lipoparticles to Deliver Hydrophobic/Amphiphilic Materials
(2013-08-14)
Chu, Hsueh-Liang
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Cheng, Tsai-Mu
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Chen, Hung-Wei
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Chou, Fu-Hsuan
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Chang, Yu-Chuan
To develop a drug delivery system (DDS), it is critical to address challenging tasks such as the delivery of hydrophobic and amphiphilic compounds, cell uptake, and the metabolic fate of the drug delivery carrier. Low-density lipoprotein (LDL) has been acknowledged as the human serum transporter of natively abundant lipoparticles such as cholesterol, triacylglycerides, and lipids. Apolipoprotein B (apo B) is the only protein contained in LDL, and possesses a binding moiety for the LDL receptor that can be internalized and degraded naturally by the cell. Therefore, synthetic/reconstituting apoB lipoparticle (rABL) could be an excellent delivery carrier for hydrophobic or amphiphilic materials. Here, we synthesized rABL in vitro, using full-length apoB through a five-step solvent exchange method, and addressed its potential as a DDS. Our rABL exhibited good biocompatibility when evaluated with cytotoxicity and cell metabolic response assays, and was stable during storage in phosphate-buffered saline at 4 degrees C for several months. Furthermore, hydrophobic superparamagnetic iron oxide nanoparticles (SPIONPs) and the anticancer drug M4N (tetra-O-methyl nordihydroguaiaretic acid), used as an imaging enhancer and lipophilic drug model, respectively, were incorporated into the rABL, leading to the formation of SPIONPs- and M4N- containing rABL (SPIO@rABL and M4N@rABL, respectively). Fourier transform infrared spectroscopy suggested that rABL has a similar composition to that of LDL, and successfully incorporated SPIONPs or M4N. SPIO@rABL presented significant hepatic contrast enhancement in T-2-weighted magnetic resonance imaging in BALB/c mice, suggesting its potential application as a medical imaging contrast agent. M4N@rABL could reduce the viability of the cancer cell line A549. Interestingly, we developed solution-phase high-resolution transmission electron microscopy to observe both LDL and SPIO@rABL in the liquid state. In summary, our LDL-based DDS, rABL, has significant potential as a novel DDS for hydrophobic and amphiphilic materials, with good cell internalization properties and metabolicity.