Browsing by Author "Hayashi-Takagi, Akiko"

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Fast 3D visualization of endogenous brain signals with high-sensitivity laser scanning photothermal microscopy
(2016-05-01)
Miyazaki, Jun
;
Iida, Tadatsune
;
Tanaka, Shinji
;
Hayashi-Takagi, Akiko
;
Kasai, Haruo
A fast, high-sensitivity photothermal microscope was developed by implementing a spatially segmented balanced detection scheme into a laser scanning microscope. We confirmed a 4.9 times improvement in signal-to-noise ratio in the spatially segmented balanced detection compared with that of conventional detection. The system demonstrated simultaneous bi-modal photothermal and confocal fluorescence imaging of transgenic mouse brain tissue with a pixel dwell time of 20 mu s. The fluorescence image visualized neurons expressing yellow fluorescence proteins, while the photothermal signal detected endogenous chromophores in the mouse brain, allowing 3D visualization of the distribution of various features such as blood cells and fine structures probably due to lipids. This imaging modality was constructed using compact and cost-effective laser diodes, and will thus be widely useful in the life and medical sciences. (C) 2016 Optical Society of America
Sub-diffraction resolution pump-probe microscopy with shot-noise limited sensitivity using laser diodes
(2014-04-21)
Miyazaki, Jun
;
Tsurui, Hiromichi
;
Hayashi-Takagi, Akiko
;
Kasai, Haruo
;
Kobayashi, Takayoshi
We demonstrate the use of intensity-modulated laser diodes to implement pump-probe microscopy and achieved sub-diffraction resolution imaging with shot-noise limited sensitivity with a scheme of balanced detection. This technique has several applications for various types of induced transmission change, including excited-state absorption, ground state absorption bleaching and stimulated emission. By using our technique, biological imaging of mouse T cells and the axons of neurons in the cerebral cortex was demonstrated. (C) 2014 Optical Society of America